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Hek293 cells

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HEK293 cells are a widely used cell line derived from human embryonic kidney cells. They are a common laboratory tool for various applications, including gene expression, viral production, and protein production. HEK293 cells provide a robust and reliable system for these purposes.

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Lab products found in correlation

Rapamycin, by LC Laboratories (2 mentions) Keyhole limpet hemocyanin (klh), by Merck Group (1 mentions) Atto 590, by Merck Group (1 mentions) Ponasterone a, by Thermo Fisher Scientific (1 mentions)

2 protocols using hek293 cells

1

Recombinant FVIII Production and Purification

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Recombinant human B domain deleted (BDD) FVIII (Xyntha) was from Pfizer (New York, NY). FVIII and FIX deficient plasma was from Haematologic Technologies (Essex Junction, VT). Anti-mCD20 IgG2a subtype (clone 18B12) was purified from transfected HEK293 cells (ATUM, Newark, CA). Rapamycin was purchased from LC laboratories (Woburn, MA). Keyhole limpet hemocyanin was purchased from Sigma Aldrich (St. Louis, MO).
Biswas M., Palaschak B., Kumar S.R., Rana J, & Markusic D.M. (2020). B Cell Depletion Eliminates FVIII Memory B Cells and Enhances AAV8-coF8 Immune Tolerance Induction When Combined With Rapamycin. Frontiers in Immunology, 11, 1293.
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2

Purification and Labeling of Antibodies

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α-mCD20 IgG2a subtype (clone 18B12) was purified from transfected HEK293 cells (ATUM, Newark, CA). Rapamycin was purchased from LC Laboratories (Woburn, MA). α-mBAFF IgG1 subtype (clone Sandy-2) was purchased from AdipoGen Life Sciences (San Diego, CA). Atto-590 (catalog no. 70425) was purchased from Sigma-Aldrich. Ponasterone A, an analog of insect steroid hormone ecdysone, was purchased from Invitrogen (Carlsbad, CA).
Rana J., Herzog R.W., Muñoz-Melero M., Yamada K., Kumar S.R., Lam A.K., Markusic D.M., Duan D., Terhorst C., Byrne B.J., Corti M, & Biswas M. (2024). B cell focused transient immune suppression protocol for efficient AAV readministration to the liver. Molecular Therapy. Methods & Clinical Development, 32(1), 101216.
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