Biochemical parameters were assessed as we previously described20 (link),50 . Blood glucose levels were assessed with a reflectance meter (OneTouch UltraEasy, LifeScan, Milpitas, CA, USA). Plasma cholesterol and triglycerides were measured using the Reflotron test (catalog 10745065202 and catalog 10745049202, Roche Diagnostic Corporation, Indianapolis, IN, USA). Urinary albumin excretion was measured with the ELISA test using the Bethyl test kit (catalog E101, catalog A90-134A and catalog A90-134P, Bethyl Laboratories Inc, Montgomery, TX, USA).
Bethyl test kit
Manufactured by Fortis Life Sciences
Sourced in United States
The Bethyl test kit is a laboratory equipment product designed for conducting various immunoassays. It provides the necessary components and reagents to perform specific tests, but a detailed description of its core function is not available without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
3 protocols using bethyl test kit
1
Biochemical Parameter Assessment Protocol
2
Metabolic Biomarker Assessment in Mice
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Blood glucose levels were assessed with a reflectance meter (OneTouch UltraEasy, LifeScan, Milan, Italy). Urinary albumin excretion was measured with the ELISA test using the Bethyl test kit (catalogues E101, A90-134A, and A90-134P, Bethyl Laboratories Inc., Montgomery, TX, USA). Urinary creatinine concentration was measured using an enzymatic method with Miura one auto-analyser (I.S.E. S.r.l. Rome, Italy). Systolic blood pressure was measured with a computerised tail-cuff system in conscious mice (BP-2000 Blood Pressure Analysis System, Visitech System; Apex, White Oak, NC, USA).
3
Biomarkers of Metabolic Health
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Blood glucose levels were assessed with a reflectance meter (One-Touch UltraEasy, LifeScan, Milan, Italy). Plasma cholesterol, triglycerides and serum blood urea nitrogen were measured using the Reflotron test (Roche Diagnostic Corporation, Indianapolis, IN, USA). Urinary albumin excretion was measured with the enzyme-linked immunosorbent assay (ELISA) test using the Bethyl test kit (Bethyl Laboratories Inc., Montgomery, TX, USA). Urinary creatinine concentration was measured using the enzymatic method with a Miura One autoanalyser (I.S.E. srl, Rome, Italy).
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