Eca 109

Manufactured by Thermo Fisher Scientific

Sourced in United States, China

The Eca-109 is a compact and versatile laboratory instrument designed for electrochemical analysis. It features a potentiostat/galvanostat module that enables precise control and measurement of electrochemical parameters, such as current, voltage, and electrochemical impedance. The Eca-109 is a self-contained unit that can be used for a variety of electrochemical applications in research and testing environments.

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Lab products found in correlation

48 protocols using eca 109

PIAS3 Knockdown in ESCC Cell Line

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Human ESCC cell line ECA109 (CC-Y1150) was purchased from Shanghai Cell Bank (Shanghai Biological Sciences, Chinese Academy of Sciences, Shanghai, China). The ECA109 cells were adherent cells and were cultured in Petri dishes. After successful resuscitation, the ECA109 cells were added to a complete culture medium composed of Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA) containing 100 U/mL of penicillin and 100 µg/mL of streptomycin (Solarbio, Beijing, China) and fetal bovine serum (FBS) (Lonsera, Shanghai, China) at a ratio of 9:1, and put into a 5% carbon dioxide (CO₂), 37 ℃ incubator for routine culture, and the solution was changed the next day.
The small-interfering RNAs (siRNAs) targeting human PIAS3 were purchased from Genepharma (Shanghai, China) with the following sequences: PIAS3 siRNA-1#, 5'-GCAGGAACCCTTCTACAAA-3'; PIAS3 siRNA-2#, 5'-GGAGATCCATCAGAGAATA-3'; PIAS3 siRNA-3#, 5'-GTGATGAGATCCAATTCAT-3'. The ECA109 cells were transfected with 80 nM of siRNA using Lipofectamine 8000 (Invitrogen, CA, USA) for 48 h.

Ju Q., Shi Q., Liu C., Fu G, & Shi H. (2023). Bufalin suppresses esophageal squamous cell carcinoma progression by activating the PIAS3/STAT3 signaling pathway. Journal of Thoracic Disease, 15(4), 2141-2160.

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Establishment of radioresistant esophageal cancer cell line

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The human ESC cell lines Eca109 were purchased from the Shanghai Cell Bank (Chinese Academy of Sciences) and cultured in RPMI1640 medium (Gibco, Waltham, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, USA) at 37°C in a 5% CO₂ atmosphere.
The radioresistant cell line Eca109R was established by exposing Eca109 cells to 25 doses of 2Gy irradiation (total dose of 50 Gy), as previously reported [22 (link)]. To establish twist knockdown cells, four shRNA targeting twist mRNA and one negative control were designed (Supplementary Table 1), synthesized, and cloned separately into pYr-3.1 vector previously digested with BbsI and BamHI endonucleases. The pYr-3.1-shRNA plasmids were transfected into Eca109 or Eca109R cells with Lipofectamine 3000 according to the manufacturer's instructions (Thermo Fisher Scientific, NY). The cells were cultured in G418 (800 μg/ml) to screen for stable transfectants, and the selected twist-silenced or control cells were cultured long-term in medium supplemented with 400 μg/ml G418.

Zang C., Liu X., Li B., He Y., Jing S., He Y., Wu W., Zhang B., Ma S., Dai W., Li S, & Peng Z. (2017). IL-6/STAT3/TWIST inhibition reverses ionizing radiation-induced EMT and radioresistance in esophageal squamous carcinoma. Oncotarget, 8(7), 11228-11238.

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ESCC Cell Culture and α-Amanitin Treatment

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ESCC cell lines KYSE30 and Eca-109 were acquired from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). KYSE30 and Eca-109 cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Invitrogen) in a humidified incubator containing 5% CO₂ at 37°C. Where indicated, the ESCC cells were treated with 50 μM α-amanitin (Sigma-Aldrich, Saint Louis, MO, USA) for the indicated time to inhibit RNA polymerase II-mediated transcription.

Lin J., Chen Z., Wu S., Huang W., Chen F, & Huang Z. (2019). An NF90/long noncoding RNA-LET/miR-548k feedback amplification loop controls esophageal squamous cell carcinoma progression. Journal of Cancer, 10(21), 5139-5152.

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Esophageal Cell Line Maintenance and Manipulation

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Human normal esophageal cell line HEEC (Het-1A, ATCC® CRL-2692™) was obtained from the America Type Culture Collection in May 2017, and two human esophageal carcinoma cell lines, KYSE-150 (TCHu236) and Eca-109 (TCHu69), were obtained from Shanghai Institute of Biological Sciences, Chinese Academy of Sciences in May 2017. These cell lines have been authenticated by short-tandem repeat analyses. They are free of mycoplasma contamination.
The cells were maintained in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% Penicillin-Streptomycin (10,000 U/mL) (Invitrogen). For cell transfection, siRNAs including DNMT1 siRNA, BCAT1 siRNA, scrambled siRNA (NC siRNA), miR-124-3p mimics and its negative control (NC mimics) were supplied by Ribobio Company (Guangzhou, China) and pcDNA-BCAT1(OE-BCAT1) or pcDNA-negative (OE-NC) were constructed using pcDNA3.1(+) vector. KYSE-150 and Eca-109 cells grown in 6-well cell plates (1 × 10⁶) were transfected with siRNA or pcDNA3.1vector using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction. After 6 h, the transfection solution was replaced by complete medium.
For drug treatment, KYSE-150 or Eca109 cells were treated with 5 μM 5-Azacitidine (5-Aza, Sigma-Aldrich, Saint Louis, MO, USA), which is the inhibitor of DNMT1.

Zeng B., Zhang X., Zhao J., Wei Z., Zhu H., Fu M., Zou D., Feng Y., Luo H, & Lei Y. (2019). The role of DNMT1/hsa-miR-124-3p/BCAT1 pathway in regulating growth and invasion of esophageal squamous cell carcinoma. BMC Cancer, 19, 609.

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Esophageal Squamous Cell Carcinoma Cell Lines

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The normal esophageal epithelial cell line (NE3), originally developed at Hong Kong University, was obtained from Professor Li Fu (Department of Pharmacology, Shenzhen University). Other human ESCC cell lines, including Eca-109 (RRID: CVCL_6898), KYSE-30 (RRID: CVCL_1351), KYSE-70 (RRID: CVCL_1356), KYSE-180 (RRID: CVCL_1349), KYSE-450 (RRID: CVCL_1353), and TE1 (RRID: CVCL_1759) were purchased from the Cobioer Biosciences Co., Nanjing, China. NE3, Eca-109, KYSE30, KYSE70, KYSE180, KYSE450, and TE1 cells were maintained in 10% FCS-RPMI-1640 medium (Invitrogen, Shanghai, China). NE3 cells were cultured in EpiCM 4101 medium (ScienCell, USA). The cells were cultured in humidified atmosphere of 5% CO₂ at 37°C. All cell lines were authenticated by matching the short-tandem repeat (STR) DNA profiles of the cell to the corresponding standard STR in the database of ATCC (the American Type Culture Collection) and DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen). All cells were routinely tested and found negative for mycoplasma.

TIAN L., HUANG Y., ZHANG B., SONG Y., YANG L., CHEN Q., WANG Z., WANG Y., HE Q., YANG W., YU S., LU T., LIU Z., GAO K., FAN X., SONG J, & ZHAI R. (2023). Targeting LncRNA LLNLR-299G3.1 with antisense oligonucleotide inhibits malignancy of esophageal squamous cell carcinoma cells in vitro and in vivo. Oncology Research, 31(4), 463-479.

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Esophageal Cancer Cell Line Transfection

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Eca-109 human esophageal cancer cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). KYSE-450 human esophageal cancer cells were purchased from the Beijing Institute for Cancer Research (Beijing Cancer Hospital, Beijing, China). All cells were maintained according to the manufacturer's protocols. Briefly, the cells were cultured at a density of 4×10⁴ in Roswell Park Memorial Institute-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 5% fetal bovine serum (Hangzhou Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) and 100 µ/ml penicillin/streptomycin in six-well plates in a 5% CO₂ humidified incubator at 37°C. After achieving 80% confluence, the Eca-109, and KYSE-450 cells were pretreated with LY294002 (10 µM), PD98059 (50 µM), U0126 (10 µM), bisindolylmaleimide I (4 µM), SB203580 (10 µM), AG-490 (10 µM), KN-62 (10 µM) and FK-506 (10 µM) for 1 h at 37°C, followed by transfection with pEGFP-N1-LRIG1 or pEGFP-N1 (control) plasmids using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h.

Jiang X, & Li H. (2016). Overexpression of LRIG1 regulates PTEN via MAPK/MEK signaling pathway in esophageal squamous cell carcinoma. Experimental and Therapeutic Medicine, 12(4), 2045-2052.

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Silencing FAM60A in Esophageal Cancer Cells

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The human esophageal squamous cell carcinoma cell lines Eca-109 and TE-13 were purchased from the Chinese Academy of Science (Shanghai, China). The cells were maintained in DMEM (Dulbecco’s Modified Eagle’s Medium, GIBCO) with 10% FBS (fetal bovine serum, GIBCO), 100 U/ml penicillin and 100 mg/ml streptomycin (KeyGEN, Nanjing, China) at 37 °C under a humidified atmosphere of 5% CO₂. Eca-109 and TE-13 cells were transfected with small interfering RNAs (siRNAs) or negative control sequences using iMAX (Invitrogen, Shanghai, China). Three sequences were designed and the sequences were as follows: siRNA-1 for FAM60A: sense 5′-GCAACCAGAUCAGUAAACUTT-3′, antisense 5′-AGUUUACUGAUCUGGUUGCTT-3′; siRNA-2 for FAM60A: sense 5′-CUGGAAUCAUGUGGUAGAUTT-3′, antisense 5′-AUCUACCACAUGAUUCCAGTT-3′; siRNA-3 for FAM60A: sense 5′-CUGACAGUAAACGCUAUGATT-3′, antisense 5′-UCAUAGCGUUUACUGUCAGTT-3′.

Dong G., Mao Q., Yu D., Zhang Y., Qiu M., Dong G., Chen Q., Xia W., Wang J., Xu L, & Jiang F. (2017). Integrative analysis of copy number and transcriptional expression profiles in esophageal cancer to identify a novel driver gene for therapy. Scientific Reports, 7, 42060.

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Modulation of Esophageal Cancer Cell Lines

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Esophageal epithelial cells HET‐1A and ESCC cells (Eca‐109 and TE‐1) were purchased from Chuan Qiu Biotechnology (Shanghai, China) and grown in DMEM medium (Genetimes) with 10% FBS (Genetimes). All culture dishes were placed at 37°C with 5% CO₂. The small interfering RNA (siRNA) of circ_0003340 (si‐circ_0003340), short hairpin RNA (shRNA) of circ_0003340 (sh‐circ_0003340), pCD‐ciR‐circ_0003340, miR‐940 mimic (miR‐940), miR‐940 inhibitor (anti‐miR‐940), pcDNA‐PRKAA1 (PRKAA1) and the corresponding controls (NC) including si‐NC, sh‐NC, empty pCD‐ciR and pcDNA, miR‐NC, and anti‐miR‐NC were synthesized by GenePharma and transfected into Eca‐109 and TE‐1 cells under the application of Lipofectamine 2000 (Invitrogen) in compliance with the manufacturer's instructions.

Guan X., Guan X., Wang Y., Lan T., Cheng T., Cui Y, & Xu H. (2022). Circ_0003340 downregulation mitigates esophageal squamous cell carcinoma progression by targeting miR‐940/PRKAA1 axis. Thoracic Cancer, 13(8), 1164-1175.

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Culturing Human Esophageal Cancer Cell Lines

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Human esophageal carcinoma cell lines TE-1 and Eca109 were purchased from Cell Bank of Chinese Academy of Sciences, Shanghai, China. Human esophageal carcinoma cell lines KYSE150 and KYSE510 were kindly provided by Dr. Qian Tao from The Chinese University of Hong Kong, HongKong, China. Immortalized human keratinocyte cell line HaCaT derived from human adult trunk skin was previous described [27 (link),28 (link)]. TE-1, Eca109, KYSE150 and KYSE510 cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 mg/ml streptomycin. HaCaT was cultured in DMEM medium (Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum and antibiotics as described above. All cell lines were incubated at 37˚C in a humidified atmosphere containing 5% CO2.

Liu H., Yang J., Yuan Y., Xia Z., Chen M., Xie L., Ma X., Wang J., Ouyang S., Wu Q., Yu F., Zhou X., Yang Y., Cao Y., Hu J, & Yin B. (2014). Regulation of Mcl-1 by constitutive activation of NF-kappaB contributes to cell viability in human esophageal squamous cell carcinoma cells. BMC Cancer, 14, 98.

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Esophageal Cancer Cell Culture and Manipulation

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The normal human esophageal epithelial cell line (HEEC; catalog: ZQ0989) was purchased from Zhongqiaoxinzhou (China), the EC9706 (catalog: 3,567) cell line was obtained from TOKU-E (USA), and the human Eca-109 (catalog: 1101HUM-PUMC000246), TE-1 (catalog: 1101HUM-PUMC000986), and KYSE150 (catalog: 3101HUMTCHu236) cell lines were purchased from Cell Culture Center of the Chinese Academy of Medical Sciences. All the cells above were cultured in RPMI-1640 (Gibco, USA) complete culture medium containing 10% fetal bovine serum (Gibco) at 37°C in 5% CO₂.
The TE-1 and Eca-109 cells were transfected with 2 μg of pcDNA3.1-HOXA10 or empty pcDNA3.1 by using Lipofectamine 2000 (Invitrogen, USA). The siRNAHOXA10 and its corresponding control siNC were synthesized by Ribobio (Guangzhou, China). For HOXA10 knockdown, the Eca-109 and TE-1 cells were treated with siHOXA10 or siNC, respectively.

Jiang L, & Yang Q. (2022). HOXA10 enhances cell proliferation and suppresses apoptosis in esophageal cancer via activating p38/ERK signaling pathway. Open Medicine, 17(1), 1750-1759.

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