Elisa kit for secretory immunoglobulin a

Total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL)-cholesterol, triglycerides and free fatty acids were measured in mouse serum using Hitachi 7180 auto-analyzers at the Nagahama Research Institute (Oriental Yeast Co., Ltd., Shiga, Japan). Enzyme-linked immunosorbent assay (ELISA) was used to measure serum insulin (mouse insulin ELISA kit, Shibayagi Co., Ltd., Gunma, Japan) and sIgA concentrations (ELISA Kit for Secretory Immunoglobulin A, Cloud-Clone Corp., Texas, USA). A 2:1 (v/v) chloroform-methanol solution was used to extract lipids from the liver [21 (link)], which were then dissolved in isopropanol containing 10% polyoxyethylene octylphenyl ether (Triton X-100, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). Cholesterol and triglyceride concentration in the lipid extracts were measured enzymatically using the Cholesterol E-test and Triglyceride E-test, respectively (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).

Hitachi 7180 auto-analyzers were used to analyze serum total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterols, triglycerides, non-esterified fatty acids (NEFA), and ketone body concentrations at Oriental Yeast Co., Ltd. (Shiga, Japan). Enzyme-linked immunosorbent assay (ELISA) was used to measure serum insulin (mouse insulin ELISA kit, Shibayagi Co., Ltd., Gunma, Japan) and sIgA concentrations (ELISA Kit for Secretory Immunoglobulin A, Cloud-Clone Corp., Katy, TX, USA). Hepatic cholesterol and triglyceride levels were analyzed according to a previous report [20 (link),25 (link)].