Hep 2

Manufactured by BNCC

Sourced in China

The Hep-2 is a laboratory equipment used for cell culture analysis. It is designed to provide a controlled environment for the growth and observation of Hep-2 cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Tu177, by BNCC (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Nhbec, by BNCC (2 mentions) Amc hn 8, by BNCC (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Hep 2, by Thermo Fisher Scientific (1 mentions) Hlecs, by BNCC (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions) Tu686, by BNCC (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Tu212, by BNCC (1 mentions) Hek293t cell line, by American Type Culture Collection (1 mentions)

6 protocols using hep 2

Human Oral Cancer Cell Interactions

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The human oral squamous cell carcinoma cell lines Hep-2 and human lymphatic endothelial cells (hLECs) were obtained from BeNa Culture Collection (Hangzhou, Zhejiang, China). hLEC were cultured with 85% RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) and 15% FBS (Atlas Biologicals), and Hep-2 were maintained in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) and 10% FBS at 37 °C in a humidified atmosphere containing 5% CO₂.
The hLEC cells were plated in a six-well plate and treated with EBM-2 or Hep-2 conditioned medium for 48 h. Then the cells were collected and used to extract protein and RNA for correlation detection (western blot analysis and qRT-PCR).

Zhang C., Zhu M., Wang W., Chen D., Chen S, & Zheng H. (2019). TNF-α promotes tumor lymph angiogenesis in head and neck squamous cell carcinoma through regulation of ERK3. Translational Cancer Research, 8(6), 2439-2448.

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Transfecting Laryngeal Cancer Cells

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Human laryngeal cancer cell lines TU177, Hep-2 were purchased from Bena Culture Collection (Beijing, China). All cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) with 10% Fetal Bovine Serum (FBS; Gibco, USA) at 37 °C and 5%CO₂. The cells were transfected with prognosis related-lncRNA using Lipofectamine™ 3000 (Thermo Fisher Scientific, USA).

Shi Y., Yang D, & Qin Y. (2021). Identifying prognostic lncRNAs based on a ceRNA regulatory network in laryngeal squamous cell carcinoma. BMC Cancer, 21, 705.

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Establishment of Cisplatin-Resistant Hep-2 Cell Line

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Human epithelial type 2 (Hep-2) was purchased from BeNa Culture Collection Co., Ltd. (Beijing, China), and the cells were cultured with Dulbecco's modified Eagle's medium (DMEM) including 10% fetal bovine serum (FBS) purchased from Procell Life Science&Technology Co., Ltd. (Wuhan, China). The cells were cultured at 37°C in a humidified incubator with 5% CO₂. Trypsinase solution (0.25%) (HyClone Logan, State of Utah, USA) was used to obtain adherent cells. Hep-2 cells were cultured; the cells in the culture medium contained cisplatin to establish cisplatin-resistant cells. The concentration of cisplatin was gradually increased (0.5, 1, 1.5, and 2 μM). The cells were maintained in each concentration of cisplatin for a period of 3 months.

Li G, & Cheng Z. (2021). miR-339-5p Inhibits Autophagy to Reduce the Resistance of Laryngeal Carcinoma on Cisplatin via Targeting TAK1. BioMed Research International, 2021, 9938515.

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Comparative Analysis of LSCC Cell Lines

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Two LSCC cell lines (AMC-HN-8 and HEP-2) and the normal human bronchial epithelial cell (NHBEC) line were purchased from Bena Culture Collection. All the cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum. Twenty pairs of primary LSCC paraneoplastic and carcinoma tissues were pooled from Beijing Tiantan Hospital, Affiliated to Capital Medical University. All patients were pathologically confirmed, and fresh tissue was collected from them immediately after surgery and frozen in liquid nitrogen. Tissue collection was approved by the ethical review committee at Beijing Tiantan Hospital, Affiliated to Capital Medical University.

Bai J., Liu X., Zhang S., Dang S., Zhang Y, & Zhang G. (2022). Expression of TRIM44 and its correlation with TLR4 in laryngeal squamous cell carcinoma. Cellular and molecular biology (Noisy-le-Grand, France), 68(6).

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LSCC Cell Lines and Tissue Samples

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Four LSCC cell lines (AMC-HN-8, HEP-2, TU-212 and TU-686) and the normal bronchial epithelial cell line NHBEC were purchased from Bena Culture Collection (Beijing, China). HEK293T cell line was purchased from American Type Culture Collection (ATCC) (Gaithersburg, MD, USA). All cell lines were cultured in DMEM medium (HyClone, Logan, Utah, USA) containing 10% fetal bovine serum (Biological Industries, Beit HaEmek, Israel) . Thirty pairs of primary LSCC paracancerous and cancerous tissues were collected from Chengde Central Hospital (Chengde, Hebei, China). All patients were pathologically confirmed, and the fresh tissues were immediately collected and frozen in liquid nitrogen after surgery. The collection of tissues was approved by the Review and Ethics committee of Chengde Central Hospital. Nuciferine (Purity, 99.49%) and other 49 natural products used in this study were all obtained from Selleck Chemicals (Houston, Texas, USA).

Li Q.H., Sui L.P., Zhao Y.H., Chen B.G., Li J., Ma Z.H., Hu Z.H., Tang Y.L, & Guo Y.X. (2021). Tripartite Motif-Containing 44 is Involved in the Tumorigenesis of Laryngeal Squamous Cell Carcinoma, and its Expression is Downregulated by Nuciferine. The Tohoku journal of experimental medicine, 254(1).

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Transfection of Laryngeal Cancer Cells

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Human laryngeal cancer cell lines TU177, Hep-2 were purchased from Bena Culture Collection (Beijing, China). All cells were cultured in Dulbecco's Modi ed Eagle Medium (DMEM; Gibco, USA) with 10% Fetal Bovine Serum (FBS; Gibco, USA) at 37℃ and 5%CO 2 . The cells were transfected with prognosis related-lncRNA using Lipofectamine™ 3000 (Thermo Fisher Scienti c, USA).

Shi Y., Yang D., & Qin Y. (2021). Identifying Prognostic Lncrnas Based on A ceRNA Regulatory Network in Laryngeal Squamous Cell Carcinoma.

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