Endofectin max reagent

Manufactured by GeneCopoeia

Sourced in China

EndoFectin™Max Reagent is a transfection reagent designed for efficient delivery of nucleic acids into a variety of cell types, including difficult-to-transfect cells. It is optimized for high transfection efficiency and low cytotoxicity.

Automatically generated - may contain errors

Lab products found in correlation

Gel imager, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Sartorius (1 mentions) Inverted fluorescence microscope, by Zeiss (1 mentions) Surescript first strand cdna synthesis kit, by GeneCopoeia (1 mentions) Uv spectrophotometer, by Allsheng (1 mentions) Oro staining kit, by Solarbio (1 mentions) Masson trichrome staining reagent, by Solarbio (1 mentions) Immobilon western hrp substrate luminol reagent, by Affinity Biosciences (1 mentions) Acrylamide bis solution, by Solarbio (1 mentions) Hieff quick exosome isolation kit plus, by Yeasen (1 mentions) Hematoxylin, by Solarbio (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rwpe 1, by Thermo Fisher Scientific (1 mentions) Sirnas, by GenePharma (1 mentions) Rwpe 1, by American Type Culture Collection (1 mentions)

2 protocols using endofectin max reagent

Adipocyte Differentiation Protocol

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Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).

Liang C., Gao S., Gao J., Xu Y, & Li Q. (2023). Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH. Scientific Reports, 13, 18431.

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Prostate Cell Line Culture and Transfection

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Human prostate cell lines (LNCaP, DU145, PC3 and 22RV1) and Human prostate epithelial cell line RWPE-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). 22RV1, LNCaP and DU145 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (PS; Sangon, Shanghai, China). PC3 cells were cultured in F-12 medium containing 10% FBS and 1% PS. RWPE-1 cells were cultured in Keratinocyte-SFM (K-sfm; Gibco) medium with Bovine Pituitary Extract (20–30 μg/mL) and rEGF (0.1–0.2 ng/mL) containing 10% FBS and 1% PS. The cells were placed in an incubator at 37°C with 5% CO₂. The cells were transfected at approximately 60% confluence with synthesized small interfering RNAs (siRNAs) (100 nmol/L; GenePharma, Shanghai, China) targeting circHIPK3 using EndoFectin™-Max reagent (GeneCopoeia Inc., Rockville, MD, USA).

Liu F., Fan Y., Ou L., Li T., Fan J., Duan L., Yang J., Luo C, & Wu X. (2020). CircHIPK3 Facilitates the G2/M Transition in Prostate Cancer Cells by Sponging miR-338-3p. OncoTargets and therapy, 13, 4545-4558.

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