The largest database of trusted experimental protocols

2 protocols using endofectin max reagent

1

Adipocyte Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).
+ Open protocol
+ Expand
2

Prostate Cell Line Culture and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human prostate cell lines (LNCaP, DU145, PC3 and 22RV1) and Human prostate epithelial cell line RWPE-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). 22RV1, LNCaP and DU145 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (PS; Sangon, Shanghai, China). PC3 cells were cultured in F-12 medium containing 10% FBS and 1% PS. RWPE-1 cells were cultured in Keratinocyte-SFM (K-sfm; Gibco) medium with Bovine Pituitary Extract (20–30 μg/mL) and rEGF (0.1–0.2 ng/mL) containing 10% FBS and 1% PS. The cells were placed in an incubator at 37°C with 5% CO2. The cells were transfected at approximately 60% confluence with synthesized small interfering RNAs (siRNAs) (100 nmol/L; GenePharma, Shanghai, China) targeting circHIPK3 using EndoFectin™-Max reagent (GeneCopoeia Inc., Rockville, MD, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!