Abi prism 3500 1 genetic analyzer

Cells were authenticated according to the protocol of the ATCC (Manassas VA, USA) based on 17 STR loci plus the gender-determining locus, amelogenin. Samples were processed with an ABI Prism 3500 × 1 Genetic Analyzer, and data were analyzed using GeneMapper IDX v.1.2 software (Applied Biosystems). Appropriate positive and negative controls were used, and the results were confirmed against ATCC and Deutsche Sammlung von Mikroorganismen und Zelkulturen (DSMZ) databases for each sample submitted (ATCC Sales Order No. SO0146233).

Approximately 1 × 10⁴ cells were used for Short tandem repeats (STR) profiling of 17 STR loci plus the gender-determining locus, amelogenin. (the American Type Culture Collection (ATCC), Manassas VA, USA). An ABI Prism 3500 × 1 Genetic Analyzer was used to process the samples and GeneMapper IDX v.1.2 (Applied Biosystems, Foster City, CA, USA) was used for data analysis. Appropriate positive and negative controls were used as internal standards, and the results were searched against ATCC and Deutsche Sammlung von Mikroorganismenund Zelkulturen (DSMZ) databases for each sample (ATCC sales order no. SO0146233).