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Anti mouse nk1.1 pk136

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Anti-mouse NK1.1 (PK136) is a mouse monoclonal antibody that recognizes the NK1.1 antigen expressed on natural killer (NK) cells and a subset of T cells in mice. The NK1.1 antigen is a type II transmembrane glycoprotein that serves as a marker for murine NK cells.

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Lab products found in correlation

Anti mouse eomes dan11mag, by Thermo Fisher Scientific (1 mentions) Recombinant mouse il 12, by R&D Systems (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

Spelling variants of this product

Anti-NK1.1 (33 citations) NK1.1 (PK136) (26 citations) Anti-NK1.1 (PK136, (14 citations) Anti-mouse NK1.1 (7 citations)

2 protocols using anti mouse nk1.1 pk136

1

Comprehensive Immunophenotyping of Mouse Cells

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Anti-mouse CD4 (GK1.5), anti-mouse CD8a (53-6.7), anti-mouse CD103 (2E7), anti-mouse CD127 (A7R34), anti-mouse CD11b (M1/70), anti-mouse granzyme C (SFC1D8), anti-mouse Notch1 (HMN1-12) and anti-mouse Notch2 (HMN2-35), anti-mouse CD117 (c-kit, ACK2), anti-mouse Ly-6A/E (Sca-1, D7), and anti-mouse TCRδ (GL3) antibodies were purchased from Biolegend (San Diego, CA). Anti-mouse Eomes (Dan11mag) and anti-mouse/human T-bet (4B10) were purchased from eBioscience (San Diego, CA). Anti-mouse CD3ε (145-2C11), anti-mouse PLZF (R17-809) and PE-CF594 Streptavidin (RUO) were from BD Biosciences (San Jose, CA). Anti-mouse NK1.1 (PK136) was from Biolegend (San Diego, CA) and eBioscience (San Diego, CA). Mouse lineage cell detection cocktail-biotin (#130-092-613) is purchased from Miltenyi Biotech.
Song E.H., Xu M., Yang J., Xiao Y., Griffith A.V, & Xiong N. (2022). DL4-derived Notch signals differentially regulate thymic generation of skin-homing CCR10+NK1.1+ innate lymphoid cells at neonatal and adult stages. Journal of immunology (Baltimore, Md. : 1950).
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2

NK Cell Stimulation Assay

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104 purified splenic Ly49Hlo or
Ly49Hhi NK cells were stimulated for 4 hours in RPMI
containing 10% fetal bovine serum with 20 ng/mL recombinant mouse IL-12
(R&D Systems) plus 10 ng/mL IL-18 (MBL), 10 ng/mL PMA (Sigma) plus 1
g/mL Ionomycin (Sigma), or 25 g/mL of plate-bound anti-mouse NK1.1 (PK136,
BioLegend) or anti-mouse Ly49H (3D10, BioLegend). Cells were cultured in
media alone as a negative control.
Adams N.M., Geary C.D., Santosa E.K., Lumaquin D., Le Luduec J.B., Sottile R., van der Ploeg K., Hsu J., Whitlock B.M., Jackson B.T., Weizman O.E., Huse M., Hsu K.C, & Sun J.C. (2019). Cytomegalovirus infection drives avidity selection of natural killer cells. Immunity, 50(6), 1381-1390.e5.
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