SEC‐HPLC was performed on a Shimadzu LC‐20A Prominence system equipped with a diode array detector with a Superdex 200 Increase 10/300 GL column (Cytiva), in PBS with 200 mM NaCl at a constant flow rate of 0.75 ml min–1. For analysis 20 μg protein at about 1 mg ml–1 were loaded. Column calibration was performed with a set of molecular mass standards ranging from 1.3 to 670 kDa (Bio‐RAD).
Lc 20a prominence system
Manufactured by Cytiva
Sourced in Japan
The LC-20A Prominence system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features a modular design, enabling customization to meet specific laboratory requirements. The system includes a solvent delivery unit, an autosampler, a column oven, and a UV/Vis detector, providing reliable and consistent performance for a wide range of HPLC applications.
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Lab products found in correlation
2 protocols using lc 20a prominence system
1
SEC-HPLC Protein Size Characterization
2
HPLC-SEC Analysis of Protein Samples
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A Shimadzu (Kyoto, Japan) LC-20A Prominence system equipped with a diode array detector was used to perform HPLC-SEC with a Superdex 200 Increase 10/300 GL column (Cytiva, Marlborough, MA, USA) in PBS with 200 mM NaCl as the mobile phase buffer. A total of 20 µg of protein at about 1 mg/mL was loaded on the column and eluted at a constant flow rate of 0.75 mL/min. Column calibration was performed with a set of molecular weight standards ranging from 670 to 1.3 kDa (Bio-RAD, Hercules, CA, USA).
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