670 ir spectrometer

The infrared spectra of the analyzed samples were measured using 670-IR spectrometer (Agilent, USA). To ensure 20-fold internal reflection of the absorbed beam, Attenuated Total Reflectance (ATR) attachment was used in the form of a ZnSe crystal with adequate geometry (truncated at 45°). Sixteen scans were registered during the measurement, and subsequently, the program averaged the results for all spectra. Prior to the measurement, the ZnSe crystal was cleaned using ultraclear solvents (Sigma-Aldrich). Before (1 h) and during the experiment, the measurement chamber was kept in an inert N₂ atmosphere. Spectral measurements were recorded in the region from 700 to 3800 cm⁻¹ at a resolution of 1 cm⁻¹. The measurements were taken at the Central Apparatus Laboratory of the University of Life Sciences in Lublin. The spectra were analyzed and processed using Grams/AI software developed by ThermoGalactic Industries (USA). All the spectra were measured at 23 °C.

Measurements of infrared spectra for the analyzed samples were conducted with the use of a 670-IR spectrometer (Agilent, Santa Clara, CA, USA). An ATR (attenuated total reflection) attachment was used in the form of a ZnSe crystal with adequate geometry (truncated at 45°) to ensure 20-fold internal reflection of the absorbed beam. During the measurement, 24 scans were registered, and, subsequently, the programme averaged the results for all spectra. Prior to the measurement, the ZnSe crystal was cleaned using ultra-clear solvents by Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Prior to (1 h) and during the experiment, the measurement chamber was kept in an inert N₂ atmosphere. Spectral measurements were recorded in the region from 550 to 3800 cm⁻¹ at the resolution of 0.5 cm⁻¹. The measurements were conducted at the Department of Biophysics Laboratory of the University of Life Sciences in Lublin. The spectra were analyzed and processed with the use of the Grams/AI software (version Suite 9.3) by ThermoGalactic Industries (Waltham, MA, USA). The spectra were normalized at the wave number of 1025 cm⁻¹ (for easier analysis). All the spectra were measured at room temperature.

Infrared spectra measurements for the analyzed samples were conducted with the 670-IR spectrometer (Agilent, Santa Clara, CA, USA). An ATR (attenuated total reflection) attachment was used in the form of a ZnSe crystal with adequate geometry (truncated at 45°) to ensure 20-fold internal reflection of the absorbed beam. During the measurement, 16 scans were registered and, subsequently, the program averaged the results for all spectra. Prior to the measurement, the ZnSe crystal was cleaned using ultra-clear solvents by Sigma-Aldrich. Prior to (1 h) and during the experiment, the measurement chamber was kept in an inert N₂ atmosphere. Spectral measurements were recorded in the region from 500 to 4000 cm⁻¹ at the resolution of 1 cm⁻¹. The measurements were conducted at the Central Apparatus Laboratory of the University of Life Sciences in Lublin. The spectra were analyzed and processed with the Grams/AI software by ThermoGalactic Industries (Waltham, MA, USA). All the spectra were measured at 23 °C.

Measurements of infrared spectra for 14 analysed samples (11 honey powders and 3 basic materials: honey, maltodextrin, and milk powder) were conducted with the use of a 670-IR spectrometer (Agilent, Santa Clara, CA, USA) at 23 °C. An ATR (Attenuated Total Reflection) attachment was used in the form of a ZnSe crystal with adequate geometry (truncated at 45°) to ensure 20-fold internal reflection of the absorbed beam. The powders were pressed onto the crystal surface. During the measurement, 16 scans were registered and subsequently, the programme averaged the results for all spectra. Prior to the measurement, the ZnSe crystal was cleaned using ultra-pure organic solvents by Sigma-Aldrich (Darmstadt, Germany). Prior to (1h) and during the experiment, the measurement chamber was kept in an inert N₂ atmosphere. Spectral measurements were recorded in the region from 500 to 4000 cm⁻¹ at the resolution of 1 cm⁻¹.

The aggregation kinetics of TTR-WT and TTR-L55P were studied by FTIR measurements in attenuated total reflection (ATR) as previously reported^{11 (link),64 (link)}. In particular, 3 µL of sample aliquots were deposited on the single reflection diamond crystal of the ATR device and the FTIR spectra were collected after solvent evaporation. A 670-IR spectrometer (Varian Australia, Mulgrave, Australia) equipped with a nitrogen-cooled mercury cadmium telluride detector was used under the following conditions: 2.0 cm⁻¹ spectral resolution, 25 kHz scan speed, 1000 scan coadditions, and triangular apodization. The second derivatives of the measured spectra were obtained after Savitsky-Golay smoothing using the Resolutions-Pro software (Varian Australia).