SEC analysis was performed using a Varian 390-LC MDS system equipped with a PL-AS RT/MT autosampler, a PL-gel 3 μm (50 x 7.5 mm) guard column, two PL-gel 5 μm (300 x 7.5 mm) mixed-D columns using DMF with 5 mM NH4BF4 at 50 °C as eluent at a flow rate of 1.0 mL.min-1. The SEC system was equipped with ultraviolet (UV)/visible (set at 280 and 461 nm) and differential refractive index (DRI) detectors. Narrow molecular weight PMMA standard (200 - 1.0 x 106 g.mol-1) were used for calibration using a second order polynomial fit. NMR spectroscopy (1H, 13C) was conducted on a Bruker DPX-300, Bruker DRX-500 or Bruker AV III 600 spectrometer using deuterated chloroform or deuterated methanol as solvent. All chemical shifts are reported in ppm (δ) relative to the solvent used. FTIR spectra were acquired using a Bruker Vector 22 FTIR spectrometer with a Golden Gate diamond attenuated total reflection cell. A total of 64 scans were collected on samples in their native state. Microscopy was performed using a Zeiss LSM 880 microscope. SYTO-9 dye was imaged by excitation at 488 nm and emission at 530 nm for green fluorescence. Propidium iodide was imaged by excitation at 561 nm and emission at 646 nm for red fluorescence.
390 lc mds system
Manufactured by Agilent Technologies
The 390-LC MDS system is a liquid chromatography-mass spectrometry (LC-MS) instrument designed for analytical and preparative applications. It integrates a high-performance liquid chromatography (HPLC) system with a mass spectrometer to provide sensitive and accurate analysis of a wide range of compounds.
Automatically generated - may contain errors
Lab products found in correlation
Dpx 300, by Bruker (4 mentions)
Vector 22 ftir spectrometer, by Bruker (3 mentions)
Rt mt autosampler, by Agilent Technologies (3 mentions)
Lsm 880 microscope, by Zeiss (2 mentions)
Aviii 600, by Bruker (2 mentions)
Drx 500, by Bruker (2 mentions)
Diamond attenuated total reflection cell, by Bruker (2 mentions)
Synergy multidetection microplate reader, by Agilent Technologies (2 mentions)
Pl as rt mt auto, by Agilent Technologies (2 mentions)
Dpx 400, by Bruker (2 mentions)
Eclipse ti, by Nikon (1 mentions)
5 protocols using 390 lc mds system
1
Characterization of Polymeric Materials
2
Multimodal Characterization Workflow
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SEC analysis was performed
using a Varian 390-LC MDS system equipped with a PL-AS RT/MT autosampler,
a PL-gel 3 μm (50 × 7.5 mm) guard column, two PL-gel 5
μm (300 × 7.5 mm) mixed-D columns using DMF with 5 mM NH4BF4 at 50 °C as eluent at a flow rate of 1.0
mL.min–1. The SEC system was equipped with ultraviolet
(UV)/visible (set at 280 and 461 nm) and differential refractive index
(DRI) detectors. Narrow molecular weight PMMA standard (200–1.0
× 106 g mol–1) were used for calibration
using a second order polynomial fit. NMR spectroscopy (1H, 13C) was conducted on a Bruker DPX-300, Bruker DRX-500
or Bruker AV III 600 spectrometer using deuterated chloroform or deuterated
methanol as solvent. All chemical shifts are reported in ppm (δ)
relative to the solvent used. FTIR spectra were acquired using a Bruker
Vector 22 FTIR spectrometer with a Golden Gate diamond attenuated
total reflection cell. A total of 64 scans were collected on samples
in their native state. Microscopy was performed using a Zeiss LSM
880 microscope. SYTO-9 dye was imaged by excitation at 488 nm and
emission at 530 nm for green fluorescence. Propidium iodide was imaged
by excitation at 561 nm and emission at 646 nm for red fluorescence.
using a Varian 390-LC MDS system equipped with a PL-AS RT/MT autosampler,
a PL-gel 3 μm (50 × 7.5 mm) guard column, two PL-gel 5
μm (300 × 7.5 mm) mixed-D columns using DMF with 5 mM NH4BF4 at 50 °C as eluent at a flow rate of 1.0
mL.min–1. The SEC system was equipped with ultraviolet
(UV)/visible (set at 280 and 461 nm) and differential refractive index
(DRI) detectors. Narrow molecular weight PMMA standard (200–1.0
× 106 g mol–1) were used for calibration
using a second order polynomial fit. NMR spectroscopy (1H, 13C) was conducted on a Bruker DPX-300, Bruker DRX-500
or Bruker AV III 600 spectrometer using deuterated chloroform or deuterated
methanol as solvent. All chemical shifts are reported in ppm (δ)
relative to the solvent used. FTIR spectra were acquired using a Bruker
Vector 22 FTIR spectrometer with a Golden Gate diamond attenuated
total reflection cell. A total of 64 scans were collected on samples
in their native state. Microscopy was performed using a Zeiss LSM
880 microscope. SYTO-9 dye was imaged by excitation at 488 nm and
emission at 530 nm for green fluorescence. Propidium iodide was imaged
by excitation at 561 nm and emission at 646 nm for red fluorescence.
3
Comprehensive Characterization of Polymers
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NMR spectra were recorded on Bruker DPX-300 and DPX-400 spectrometers for 1H NMR (400 MHz) and 13C NMR (125 MHz). Chemical shifts are reported in ppm relative to the deuterated solvent resonances and spectra analysed with WIN-NMR software. GPC (DMF) was performed on a Varian 390-LC MDS system equipped with a PL-AS RT/MT auto-sampler, a PL-gel 3 μm (50 × 7.5 mm) guard column, two PL-gel 5 μm (300 × 7.5 mm) mixed-D columns equipped with a differential refractive index, using DMF (with 1 mg per mL LiBr) as the eluent with a flow rate of 1.0 mL min–1 at 50 °C. Narrow molecular weight PMMA standards (200–1.0 × 106 g mol–1) were used for calibration using a second order polynomial fit. Infrared absorption spectra were recorded on a Bruker VECTOR-22 FTIR spectrometer using a Golden Gate diamond attenuated total reflection cell. Absorbance measurements were recorded on a BioTek Synergy™ multidetection microplate reader using Gen5 1.11 multiple data collection and analysis software.
4
Comprehensive Polymer Analysis Protocol
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SEC analysis was performed
using a Varian 390-LC MDS system equipped with a PL-AS RT/MT autosampler,
a PL-gel 3 μm (50 Å∼ 7.5 mm) guard column, and two
PL-gel 5 μm (300 Å∼ 7.5 mm) mixed-D columns using
DMF with 5 mM NH4BF4 at 50 °C as eluent
at a flow rate of 1.0 mL·min–1. The SEC system
was equipped with ultraviolet (UV)/visible (set at 280 and 461 nm)
and differential refractive index (DRI) detectors. Narrow-molecular-weight
PMMA standard (200–1.0 Å∼ 106 g mol–1) was used for calibration using a second-order polynomial fit. NMR
spectroscopy (1H, 13C) was conducted on a Bruker
DPX-300 using deuterated chloroform or methanol as a solvent. Fluorescence
microscopy was performed on a Nikon Eclipse Ti, inverted wide-field
fluorescence microscope equipped with LED illumination, a 100×
1.45 NA objective, mCherry and GFP filter sets, and a 2k × 2k
sCMOS Andor camera system. Transmission electron microscopy (TEM)
was performed on a JEOL 2100 instrument at 200 kV, and images obtained
were analyzed using ImageJ software.
using a Varian 390-LC MDS system equipped with a PL-AS RT/MT autosampler,
a PL-gel 3 μm (50 Å∼ 7.5 mm) guard column, and two
PL-gel 5 μm (300 Å∼ 7.5 mm) mixed-D columns using
DMF with 5 mM NH4BF4 at 50 °C as eluent
at a flow rate of 1.0 mL·min–1. The SEC system
was equipped with ultraviolet (UV)/visible (set at 280 and 461 nm)
and differential refractive index (DRI) detectors. Narrow-molecular-weight
PMMA standard (200–1.0 Å∼ 106 g mol–1) was used for calibration using a second-order polynomial fit. NMR
spectroscopy (1H, 13C) was conducted on a Bruker
DPX-300 using deuterated chloroform or methanol as a solvent. Fluorescence
microscopy was performed on a Nikon Eclipse Ti, inverted wide-field
fluorescence microscope equipped with LED illumination, a 100×
1.45 NA objective, mCherry and GFP filter sets, and a 2k × 2k
sCMOS Andor camera system. Transmission electron microscopy (TEM)
was performed on a JEOL 2100 instrument at 200 kV, and images obtained
were analyzed using ImageJ software.
5
Comprehensive Characterization of Polymers
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