2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), veratryl alcohol, catechol, veratryl alcohol, methyl red, nicotinamide adenine dinucleotide (NADH) and dichlorophenol indophenols (DCIP) were procured from HiMedia Laboratories Pvt. Ltd. (Mumbai, India). Chloranil, dimethylformamide (DMF), aniline-2-sulfonic acid and all other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA). All the chemicals used in this study were of high purity (>98%). Trypan Blue (CAS No. 72-57-1; CI No. 23850; CI Name = Direct Blue 14; Molecular Formula = C34H24N6O14S4Na4; Molecular Weight = 960.81 g∙mol−1; λmax 660 nm); λmax 660 nm), a water-soluble azo dye was procured from Sigma-Aldrich and used as received. Its chemical structure is shown in Figure 1 . Unless otherwise stated, the working concentration of dye Trypan Blue was 50 mg∙L−l.
Azo dye
Manufactured by Merck Group
Sourced in United States
Azo dyes are a class of synthetic organic dyes characterized by the presence of one or more azo groups (-N=N-) in their molecular structure. These dyes are commonly used in a variety of laboratory applications, including staining and labeling procedures.
Automatically generated - may contain errors
Lab products found in correlation
Veratryl alcohol, by HiMedia (1 mentions)
Catechol, by HiMedia (1 mentions)
Methyl red, by HiMedia (1 mentions)
Chloranil, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
2 2 azino bis, by HiMedia (1 mentions)
Nicotinamide adenine dinucleotide nadh, by HiMedia (1 mentions)
Cross linker, by Merck Group (1 mentions)
Pegda, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazolin 6 sulfonic acid, by Merck Group (1 mentions)
5 protocols using azo dye
1
Enzymatic Decolorization of Trypan Blue
2
Self-Healing Cross-Linked Gel Electrolytes
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Self-healing
cross-linked gel electrolytes
based on PEGDA were obtained through in situ UV polymerization
of precursor solutions. All the procedures described in the following
were performed in an argon-filled glovebox (MBraun, O2,
H2O < 0.5 ppm). PEGDA, cross linker, photo initiator,
and the azo dye were all purchased from Sigma-Aldrich; the liquid
electrolyte, LP30 (LiPF6 1 M in EC/DMC 50/50 v/v), was
provided by Solvionic. The UPyPEG35000UPy self-healing
unit was synthesized as described in detail in ref (17 (link)). The UPy-acrylate monomer
(UPy-MA, a comonomer shown inFigure S1 ) was synthesized as described in the following section.
cross-linked gel electrolytes
based on PEGDA were obtained through in situ UV polymerization
of precursor solutions. All the procedures described in the following
were performed in an argon-filled glovebox (MBraun, O2,
H2O < 0.5 ppm). PEGDA, cross linker, photo initiator,
and the azo dye were all purchased from Sigma-Aldrich; the liquid
electrolyte, LP30 (LiPF6 1 M in EC/DMC 50/50 v/v), was
provided by Solvionic. The UPyPEG35000UPy self-healing
unit was synthesized as described in detail in ref (17 (link)). The UPy-acrylate monomer
(UPy-MA, a comonomer shown in
3
Decolorization of Azo Dyes Protocol
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The azo dyes used in the decolorization studies were purchased from Sigma-Aldrich (USA). Their physicochemical characteristics are presented in Table S1 . 2,2-Azinobis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS); 2,6-dimethoxyphenol (DMP); 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO); gallic acid (GA); caffeic acid (CA); and vanillin (V) were purchased from Sigma-Aldrich (USA). The other chemicals were obtained from Promega (USA), Serva (Germany), Bio-Rad (USA), and POCh (Gliwice, Poland). All reagents were analytical grade. Buffers and solutions were prepared in distilled water. For proteomic analysis, ultrapure water was used.
4
Azo Dye Biotransformation Protocols
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Five Azo dyes (Sigma-Aldrich, St. Louis, USA) were used in this study. The molecular structures and absorption wavelength of these dyes were shown in Fig. S1 . Other chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Biochemical reagents and Dr. GenTLER (from Yeast) High Recovery were purchased from TaKaRa Biotechnology Co., Ltd. (Japan). Yeast Malt Extract (YME) consisted of (g/L): 3.0 yeast extract; 5.0 peptone 3.0 malt extract and 10 glucose. Minimal Saline (MS) medium consisted of (g/L): 0.05 CaCl2, 0.05 MgSO4, 1.0 KH2PO4, 0.2 (NH4)2SO4 and 20 NaCl was used, containing 10 glucose as carbon source.
5
Azo Dye Decolorization by Agricultural Waste
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Nine azo dyes (Sigma-Aldrich, St. Louis, USA) were used in this study. The names of these dyes are Reactive Green 19, Methyl Red, Methyl Orange, Reactive Blue 81, Reactive Red 120, Reactive Violet 5, Acid Orange 7, Acid Brilliant Scarlet GR, and Reactive Black 5. The chemical structure of dyes and their corresponding maximum wavelengths (λmax) are given in Fig. 9 . In this work, several agricultural wastes (rice straw, bagasse, wheat bran, rice stalk, and sorghum husk) were obtained from local farmers and industries (Zhenjiang, China) to evaluate their impacts on the AO7 dye decolorization by the developed yeast consortium. The agricultural waste extracts were prepared in the Bushnell Haas medium (5.0 mL extract of 0.5% boiled agricultural residue).![]()
Chemical structure of various azo dyes used in this study and their corresponding maximum wavelengths (λmax)
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