Semen samples were incubated with 10 µM ThT or 1 µM the luminescent-conjugated oligothiophene dye pFTAA for 15- 30 min. ThT was excited by a 405 nm laser line and the emission was collected using MBS-405 beam splitter. pFTAA was excited by a 488 nm laser line and the corresponding emission was collected using MBS-488 beam splitter. Confocal images were acquired using Plan-Apochromat 63X/1.40 oil objective lenses on a LSM710 confocal microscope (Zeiss) equipped with Zen-Software 2010 (Zeiss, Germany). The confocal pin hole was adjusted to 1 airy unit. Images were processed using the Zen-Software 2010 (Zeiss, Germany).
Zen software 2010
Manufactured by Zeiss
Sourced in Germany
The Zen-Software 2010 is a comprehensive imaging and analysis software suite developed by Zeiss. It provides a platform for managing, processing, and visualizing microscopy data. The software supports a wide range of Zeiss microscope systems and offers advanced tools for image acquisition, enhancement, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710 confocal microscope, by Zeiss (6 mentions)
Lsm 780 confocal microscope, by Zeiss (6 mentions)
Lsm 700 confocal microscope, by Zeiss (4 mentions)
Cellmask orange plasma membrane stain, by Thermo Fisher Scientific (2 mentions)
Pre coated poly l lysine glass bottom microwell dishes, by MatTek (2 mentions)
Plan apochromat 63x 1.40 oil, by Zeiss (2 mentions)
Zen blue software, by Zeiss (2 mentions)
Imager z2 microscope, by Zeiss (1 mentions)
Axiocam mrm, by Zeiss (1 mentions)
Mounting medium, by Dianova (1 mentions)
Axiovision version 4, by Zeiss (1 mentions)
Axio imager z2, by Zeiss (1 mentions)
6 protocols using zen software 2010
1
Amyloid Protein Detection in Semen
2
Amyloid Protein Detection in Semen
3
Histamine-Induced Trio Translocation
4
Histamine-Induced Trio Translocation
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HEK 293 cells were grown onto pre-coated (poly-L-lysine) glass bottom microwell dishes (MatTek Corporation). Cells were transfected with H1R1 and Trio-GFP. Next day cells were serum starved for 4 h and incubated with CellMask™ Orange plasma membrane stain (Invitrogen) according to the manufacturer’s instructions. Cells were placed in the microscope in serum-free media and stimulated with histamine. To quantify the levels of Trio-GFP in the plasma membrane, the peak fluorescence value of a line histogram covering the plasma membrane region was recorded every 30 sec and used to plot the fluorescent levels of membrane Trio over time. At least 5 different regions were quantified in a cell and averaged. Live cell imaging and quantifications were performed using an inverted Zeiss LSM 700 confocal microscope equipped with a temperature and CO2 incubator, coupled to Zen software 2010 (Carl Zeiss).
5
Quantification of Keratin Cytoskeleton Fluorescence
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Images for quantification of the keratin cytoskeleton were collected with a Zeiss LSM 780 confocal microscope equipped with 63×/1.46 NA oil immersion objective and Aryscan. The size of the pinhole was always set to 1 Airy unit. To quantify the fluorescence intensity, images were taken with a Zeiss Imager Z2 microscope equipped with ApoTome with a 40×/1.3 NA oil immersion objective and a AxioCam Mrm. Image’s with a pixel dimension of 0.161 µm × 0.161 µm were taken. Image analysis and processing were performed using Zen Software 2010 (Carl Zeiss, Inc.), Zen Blue Software (Carl Zeiss, Inc.), ImageJ and Photoshop CS4 (Adobe) software. LUT (lookup table; brightness) was adjusted using Photoshop.
6
Immunofluorescence Staining and Imaging
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Staining conditions and image processing was described previously (Seltmann et al., 2013a) . In brief, cells were fixed for 10 minutes in 4% paraformaldehyde and incubated with primary antibodies for 1 hour. All antibodies were diluted in TBS containing 1% BSA. Afterward, cells were incubated with the secondary antibody for 30 minutes and mounted with mounting medium (Dianova). Images were acquired using an AxioImager Z2 equipped with Zeiss Plan-Apochromat × 63/1.46 oil or a Zeiss LSM 780 confocal microscope with 40/1.3 NA or 63 /1.46 NA. Image analysis and processing were performed using the AxioVision version 4.8 and Zen Software 2010 (Zeiss, Jena, Germany).
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