Biotinylated bovine serum albumin

Genomic DNA of each isolate was extracted with the DNA extraction kits (QIAamp DNA mini kits; Qiagen, Hilden, Germany). Color indicator (Visual detection reagent, VDR) and DNA isothermal amplification kit were purchased from Tian-jing Huidexing. Co., Ltd. (Beijing, China). The materials, including backing card, sample pad, conjugate pad, nitrocellulose membrane (NC) and absorbent pad, were obtained from the Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Biotin-BSA (Biotinylated bovine serum albumin) and anti-FITC (rabbit anti-fluorescein antibody) were obtained from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg mL⁻¹, 100 mM borate, pH 8.5 with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, INC. (Indiana, USA).

The kit Qiagen QIAamp DNA mini kit (Qiagen, Beijing, China) was used to extract genomic DNA from fungi, bacterial and clinical. Isothermal amplification kits and colorimetric indicator (malachite green, MG) were provided by Beijing-Hai Tai Zheng Yuan Technology Co., Ltd. (Beijing, China). Dye streptavidin-coated polymer nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, United States). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were obtained from Abcam. Co., Ltd. (Shanghai, China). Polymer nanoparticle-based lateral flow biosensor (LFB) materials, including sample pad, nitrocellulose membrane (NC), absorbent pad, conjugate pad and backing card were obtained from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Primers and labeled primers (Table 2 and Supplementary Table 1) used were synthesized by Beijing-Tsingke Biotechnology Co., Ltd. (Beijing, China).

Bacterial genomic DNA extraction kits (QIAamp DNA minikits; Qiagen, Hilden, Germany) were purchased from Qiagen (Beijing, China). Universal isothermal amplification kits, a colorimetric indicator (malachite green, MG), and biotin-14-dCTP were obtained from Bei-Jing HaiTaiZhengYuan. Co., Ltd. (Beijing, China). The LFB materials, including the backing card, sample pad, absorbent pad, conjugate pad and nitrocellulose membrane (NC), were purchased from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Anti-FITC (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were purchased from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml^− 1; 100 mM borate, pH 8.5, with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, Inc. (Indiana, USA).