Dp manager imaging software

Giemsa-stained metaphase chromosomes were studied under a Carl Zeiss AxioImager.Z2 microscope, equipped with Metafer Scanning Platform (Metasystems) and a MetaSystems CoolCube digital camera. Images were processed for karyotype reconstruction with Ikaros karyotyping software (Metasystems). For C-banding, FISH and CGH methods, images from at least 20 metaphase chromosomes were analyzed using a Provis AX70 (Olympus) fluorescence microscope, equipped with a DP30BW digital camera (Olympus). All images were acquired in black and white, and later superimposed with colours in DP Manager imaging software (Olympus).

For microscopy, an Olympus System differential interference contrast microscope model BX51 (Olympus, Tokyo, Japan) and the stereomicroscope SMZ800 (Nikon, Tokyo, Japan) were used. Images were captured using a DP71 digital camera (Olympus, Tokyo, Japan) and processed using the DP manager imaging software (Olympus, Tokyo, Japan).

Coverslip culture was performed as described previously36 (link). The coverslips were stained with 1 mg/ml Hoechst 33342 (Sigma) for 10 min, briefly washed with distilled water and dipped in distilled water for 10 minutes. Then the coverslips were washed with ethanol, air-dried for 5 minutes and mounted with antifade mounting medium (H-1000; Vectashield, USA). For differential interference contrast (DIC) and fluorescence microscopy, an Olympus System microscope Model BX51 (Olympus, Japan) equipped with UPlanSApo 60× and UPlanFL 100× objective lenses (Olympus) were used. DAPI (High brightness) filter cube (Excitation filter: center wavelength 377 nm, Emission filter: center wavelength 447 nm; Olympus) and FITC filter cube (Excitation filter: center wavelength 483 nm, Emission filter: center wavelength 535 nm; Olympus) were used to observe the fluorescence of Hoechst and YFP, respectively. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus) and Photoshop CS5.1 (Adobe Systems, USA).

Images were captured using a Provis AX70 (Olympus, Tokyo, Japan) fluorescence microscope equipped with a DP30BW digital camera (Olympus Tokyo, Japan). The karyotype was arranged using Ikaros karyotyping software (Metasystems, Altlussheim, Germany). DP manager imaging software (Olympus, Tokyo, Japan) was used to capture greyscale images and to superimpose the source images with colours to visualize the results of the FISH.

For microcopy, an Olympus System microscope Model BX51 (Olympus) equipped with UPlanSApo 60X and UPlanFL 100X objective lenses (Olympus) and stereomicroscope Model SMZ800 (Nikon) were used. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus). For microscopic observation of the fungal hyphae, each strain was coverslip-cultured on a block of appropriate agar medium or incubated in liquid GMM medium. The coverslips were stained with 1 mg/ml Hoechst 33342 (Sigma-Aldrich) for labeling DNA^{2 (link)}. DAPI (high brightness) filter cubes (excitation filter: center wavelength 377 nm, emission filter: center wavelength 447 nm, Olympus) and FITC filter cubes (excitation filter: center wavelength 483 nm, emission filter: center wavelength 535 nm, Olympus) were used to observe the fluorescence of Hoechst and YFP, respectively^{50 (link)}.

The images were captured on a Provis AX70 (Olympus) fluorescence microscope with a DP30BW digital camera (Olympus) and superimposed with colour using DP Manager imaging software (Olympus). Selected Giemsa stained metaphases were karyotyped using Ikaros karyotyping software (Metasystems).

An Olympus Provis AX70 fluorescence microscope with a DP30BW digital camera was used to take grayscale images that were processed with DP manager imaging software (Olympus) to record the pattern of the telomeric repeats within the chromosomal metaphases.