Immunocytochemistry employed standard techniques [32 (link), 33 (link)]. Secondary-antibody-only controls were performed by omitting primary antibodies during incubation (see Supplementary Data ). Fluorescent imaging was performed on a Zeiss UV-LSM 510 META and a Nikon A1 confocal microscope. Sequential scanning of channels was performed to prevent false-positive co-localization. Images were quantified using ImageJ. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetra-zolium bromide (MTT) Cell Proliferation Assay Kit [34 (link)].
Uv lsm 510 meta
Manufactured by Nikon
The UV-LSM 510 META is a laser scanning microscope designed for fluorescence imaging. It features a multi-photon excitation laser and is capable of capturing high-resolution images of samples using ultraviolet wavelengths.
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Lab products found in correlation
3 protocols using uv lsm 510 meta
1
Immunocytochemistry Imaging and Cell Viability
2
Immunocytochemistry Protocol for Protein Visualization
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Immunocytochemistry was performed using previously described standard techniques [11 (link),18 (link)]. Briefly, cells were fixed with 4% paraformaldehyde solution, permeabilized with a nonionic surfactant, and blocked using bovine serum albumin or fetal bovine serum for gels. Cells were then incubated sequentially in primary and fluorescent secondary antibody solutions to allow visualization of targeted proteins by fluorescence microscopy (full details, antibody sources, and concentrations included in S1 Supporting information ). Secondary antibody-only controls were performed by omitting the primary antibody during incubation (S1 Fig ). For positive controls for immunofluorescence staining of ONPs refer to previous work [8 (link)]. Fluorescence imaging was performed on a Zeiss UV-LSM 510 META or a Nikon A1/C2 confocal microscope. Sequential scanning of channels was performed to prevent false-positive co-localization. ImageJ 1.51e 5 [33 (link)] was used to quantify images.
3
Immunocytochemistry and Cell Viability Assay
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Immunocytochemistry employed standard techniques 32 , 33 . Secondary‐antibody‐only controls were performed by omitting primary antibodies during incubation (see Supporting Information). Fluorescent imaging was performed on a Zeiss UV‐LSM 510 META and a Nikon A1 confocal microscope. Sequential scanning of channels was performed to prevent false‐positive colocalization. Images were quantified using ImageJ. Cell viability was assessed using a 3‐(4,5‐dimethylthiazol‐2yl)‐2,5 diphenyl tetra‐zolium bromide (MTT) Cell Proliferation Assay Kit 34 .
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