Western blot analyses were performed as described previously6 (link), using the following primary antibodies: anti-PLOD3 (Proteintech Group, Inc., Chicago, IL, USA); anti-HA, anti-ERK1/2, anti-p38, anti-K-RAS, anti-H-RAS (Santa Cruz Biotechnology Inc.); anti-p-anti-ERK1/2, anti-p-p38, anti-p-JNK, anti-JNK, anti-STAT3, anti-p-STAT3 (Y705), and anti-p-STAT3 (S727) (Cell Signaling Technology Inc.) antibodies. β-actin (Sigma) was used as a loading control.
Anti plod3
Manufactured by Proteintech
Sourced in United States, China
Anti-PLOD3 is a laboratory reagent used to detect the presence and expression levels of the PLOD3 protein. PLOD3 is an enzyme involved in collagen biosynthesis. This antibody can be used in various immunoassay techniques to measure PLOD3 protein levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti p p38, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Santa Cruz Biotechnology (1 mentions)
Anti kras, by Santa Cruz Biotechnology (1 mentions)
Anti hras, by Santa Cruz Biotechnology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Anti pstat3 s727, by Cell Signaling Technology (1 mentions)
Anti pstat3 y705, by Cell Signaling Technology (1 mentions)
Anti p38, by Santa Cruz Biotechnology (1 mentions)
Anti ha, by Santa Cruz Biotechnology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca assay kit, by Beyotime (1 mentions)
2 protocols using anti plod3
1
Western Blot Analysis of Protein Expression
2
Quantification of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was isolated from tissue samples using RIPA lysis buffer (Beyotime Biotechnology, China) and then quantified by the BCA assay kit (Beyotime Biotechnology, China). Each 20 μg total protein was electrophoresed on 10% SDS-polyacrylamide gel and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). The primary antibodies used were anti-PLOD3 (Proteintech, China) and anti-GAPDH (Cell Signaling Technology, USA). Membranes were then washed three times in TBST solution for 15 min each and then incubated with secondary antibodies for 1 h.
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