Matrigel stamps were applied as described in Bulger et al. (2024) (link). PDMS stamps (each containing 12×1000 μM circles) were sterilized by washing in a 70% ethanol solution and dried in a laminar flow hood. Growth factor reduced Matrigel (Corning) was diluted in DMEM/F-12 (Gibco) at 1:100 dilution and incubated on the stamps to cover the entire surface of the feature side at 37°C for 1 h. The Matrigel solution was then aspirated off the stamps, which were air-dried. Using tweezers, the Matrigel-coated surface of stamps was brought in contact with glass or plastic substrate, usually a glass 24-well plate or removable 3-chamber slide (Ibidi), and incubated on the substrate for 1 h at 37°C. The stamps were then removed and rinsed in ethanol for future use. Matrigel-printed substrates were incubated with 1% Bovine Serum Albumin (Sigma-Aldrich) in DPBS−/− at room temperature for 1 h before being stored in DPBS−/− solution at 4°C for up to 2 weeks.
Removable 3 chamber slide
Manufactured by Ibidi
The Removable 3-chamber slide is a laboratory equipment designed to facilitate microscopic observations. The product features three individual compartments that can be used for various sample preparations and analysis. The chambers are removable, allowing for easy cleaning and sample replacement.
Automatically generated - may contain errors
2 protocols using removable 3 chamber slide
1
Matrigel Micropattern Stamping Protocol
2
Matrigel Micropattern Printing Protocol
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Matrigel stamps were applied as described in Bulger et al. 2023 (link). PDMS stamps (each containing 12 × 1000uM circles) were sterilized by washing in a 70% ethanol solution and dried in a laminar flow hood. Growth factor reduced Matrigel (Corning) was diluted in DMEM/F-12 (Gibco) at 1:100 dilution and incubated on the stamps to cover the entire surface of the feature side at 37°C for 1 hour. The Matrigel solution was then aspirated off the stamps, which were airdried. Using tweezers, the Matrigel-coated surface of stamps was brought in contact with glass or plastic substrate, usually a glass 24-well plate or removable 3-chamber slide (Ibidi), and incubated on the substrate for 1hr at 37°C. The stamps were then removed and rinsed in ethanol for future use. Matrigel-printed substrates were incubated with 1% Bovine Serum Albumin (Sigma Aldrich) in DPBS−/− at room temperature for 1 hour before being stored in DPBS −/− solution at 4°C for up to 2 weeks.
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