N-BOC serinol, urea, hexamethylene diisocyanate, anhydrous N,N-dimethylformamide (DMF), poly-ε-caprolactone (80,000g/mol), n-hydroxysuccinamide (NHS), anhydrous lithium-N,N-dimethylformamide (Li-DMF), hydrogen peroxide, cobalt chloride, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCL) and 3.5kDa cellulose dialysis tube were purchased from Sigma-Aldrich (St. Louis, MO). Trifluoroacetic acid, methylene chloride, trifluoroethanol, diethyl ether and 1,1,1,3,3,3 hexafluoro-2-propanol (HFP) were purchased from Alfa Aesar (Wardhill, MA). RGD was purchased from Biomatik Inc. (Wilmington, DE, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Invitrogen (Grand Island, NY). Bovine pulmonary artery smooth muscle cells were provided by Dr. Kurt Stenmark. The hemocompatibility study kits were kindly provided by Dr. Jorge DiPaola (Department of Pediatrics-Hematology, University of Colorado Denver, Anschutz Medical Campus). Rabbit antibody for α-smooth muscle-actin (clone 1A4) and calponin (clone CALP-1) were purchased from Sigma Chemical, St. Louis, MO. Rabbit antibody for smooth muscle heavy chain myosin kindly provided by Dr. R. S. Adelstein (National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD).
1 1 1 3 3 3 hexafluoro 2 propanol hfp
Manufactured by Thermo Fisher Scientific
1,1,1,3,3,3 hexafluoro-2-propanol (HFP) is a highly fluorinated organic compound with the chemical formula (CF3)2CHOH. It is a colorless liquid with a high boiling point and low surface tension. HFP has applications as a solvent and reagent in various chemical and biotechnological processes.
Automatically generated - may contain errors
Lab products found in correlation
Cobalt chloride, by Merck Group (1 mentions)
Trifluoroethanol, by Thermo Fisher Scientific (1 mentions)
Diethyl ether, by Thermo Fisher Scientific (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by Merck Group (1 mentions)
Anhydrous n n dimethylformamide dmf, by Merck Group (1 mentions)
N boc serinol, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Urea, by Merck Group (1 mentions)
Hexamethylene diisocyanate, by Merck Group (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Tricalcium phosphate, by Merck Group (1 mentions)
Live dead viability cytotoxicity kit for mammalian cells, by Thermo Fisher Scientific (1 mentions)
Poly ε caprolactone pcl pellets, by Merck Group (1 mentions)
Bovine type b gelatin, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
2 protocols using 1 1 1 3 3 3 hexafluoro 2 propanol hfp
1
Biomimetic Polymer Scaffold Development
2
Fabrication of PCL-Gelatin Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Poly(ε-caprolactone) (PCL) pellets (Mw: 80000–90,000) and gelatin (Type B, bovine) were purchased from Sigma Aldrich (St. Luis, MO, USA). The solvent 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) were procured from Alfa Aesar (Korea). Fibronectin, ammonium bicarbonate, tricalcium phosphate were purchased from Sigma Aldrich Chemical Co. (St. Luis, MO, USA, Germany and China). Tissue culture reagents such as fetal bovine serum (FBS, Biotechnics Research, Mission Viejo, CA, USA), penicillin-streptomycin (Lonza; Seoul, Korea), 0.05% trypsin- ethylene diamine tetra-acetic acid (EDTA)-1X (Gibco-Life Technologies; Carlsbad, California, USA), and live & dead viability/cytotoxicity kit for mammalian cells (Invitrogen, Carlsbad, CA, USA) were purchased and used. Mesenchymal stem cells (Seoul National University Hospital, Seoul, Korea) were used for biocompatibility tests and distilled water (DW) was employed for all the experiments.
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