Amazon speed etd ion trap ms

Procainamide labelled samples were analyzed by HILIC-UHPLC-ESI-MS with fluorescence detection (λ_ex = 310 nm, λ_em = 370 nm), using the same UHPLC conditions as detailed above for 2-AB analysis, with the exception of column temperature (set to 40°C). ESI-MS and MS/MS detection were carried out using an amaZon speed ETD ion trap MS (Bruker, Massachusetts) as above, with the top three precursors ions selected for MS/MS fragmentation.

Procainamide labelled glycans were prepared in 0.1% TFA (v/v) in 78% acetonitrile (v/v) and desalted on-line using hydrophilic interaction liquid chromatography solid phase extraction prior to direct elution into the mass spectrometer. Samples were applied onto a HILIC trapping column (MilliporeSigma 1.50484.0001 SeQuant HPLC Guard Column with ZIC-HILIC [5 μm] Sorbent Packing Media, 5 x 0.3mm) at a flow rate of 1.5 μl/min using an UltiMate 3000 LC (Thermo Scientific, Massachusetts). The trap was washed for 4 min with 0.1% formic acid in 90% ACN (v/v) followed by elution of procainamide labelled glycans using an isocratic gradient of 0.1% formic acid in 27% ACN (v/v) for 12 min. Glycans were analysed by electrospray ionization tandem mass spectrometry (ESI-MS) on an amaZon speed ETD ion trap MS (Bruker, Massachusetts) in positive ion mode with a nitrogen flow of 10 L/min and capillary voltage of 4500 V.