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Apc alexa fluor 750 conjugated anti cd3

Manufactured by Beckman Coulter
Sourced in United States

The APC-Alexa Fluor 750-conjugated anti-CD3 is a fluorescently-labeled antibody that binds to the CD3 antigen expressed on the surface of T cells. It is a tool used in flow cytometry experiments to identify and quantify T cell populations.

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2 protocols using apc alexa fluor 750 conjugated anti cd3

1

Flow Cytometry Immunophenotyping Panel

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The following mAbs and reagents were used in this study: fluorescein isothiocyanate (FITC)-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, FITC-conjugated anti-TCR γδ and PE-conjugated 6B11 (all from Becton Dickinson, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA) and APC-Alexa Fluor 750-conjugated anti-CD3 (Beckman Coulter, Marseille, France). Cells were stained with combinations of appropriate mAbs for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA, USA).
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2

Multiparameter Flow Cytometry of Immune Cells

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The following mAbs and reagents were used in this study: fluorescein isothiocyanate (FITC)-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, FITC-conjugated anti-TCR γδ and PE-conjugated 6B11 (all from Becton Dickinson, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA) and APC-Alexa Fluor 750-conjugated anti-CD3 (Beckman Coulter, Marseille, France). Cells were stained with combinations of appropriate mAbs for 20 min at 4℃. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA, USA).
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