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Phospho ndrg1

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Phospho-NDRG1 is a lab equipment product manufactured by Cell Signaling Technology. It is an antibody that specifically recognizes the phosphorylated form of the NDRG1 protein. NDRG1 is a protein involved in various cellular processes.

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Lab products found in correlation

4e bp1, by Cell Signaling Technology (3 mentions) Rictor, by Cell Signaling Technology (3 mentions) Phospho akt, by Cell Signaling Technology (3 mentions) Ndrg1, by Cell Signaling Technology (3 mentions) Phospho s6, by Cell Signaling Technology (3 mentions) Phospho 4ebp1, by Cell Signaling Technology (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Puromycin, by Thermo Fisher Scientific (2 mentions) Propidium iodine, by Thermo Fisher Scientific (2 mentions) Phospho tyrosine, by Cell Signaling Technology (2 mentions) Antibodies against β actin, by Merck Group (2 mentions) Erlotinib, by Selleck Chemicals (2 mentions) Phospho egfr y1068, by Cell Signaling Technology (2 mentions) Phospho 4ebpl t37 46, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (1 mentions) Catalase, by Merck Group (1 mentions) Phospho prb, by Cell Signaling Technology (1 mentions) Phospho p53, by Cell Signaling Technology (1 mentions) Raptor, by Cell Signaling Technology (1 mentions) Phospho p70s6k, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

NDRG1 (28 citations) Phospho-NDRG1-Thr 346 (3 citations)

5 protocols using phospho ndrg1

1

Western Blot and Immunoprecipitation Analyses

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Western blot (WB) and immunoprecipitation (IP) analyses were performed as described previously [22 (link)]. The following antibodies were used in this study: Primary antibodies included phospho-AKT at S473 (#4060), phospho-AKT at T308 (#13038), phospho-Foxo3α at Thr32 9 (#9464), phospho-AMPKα1 at Thr172 (#2535), phospho-p70S6K at Thr389 (#9206), phospho-SIN1 at Thr86 (#14716S), phospho-NDRG1 at Thr346 (#5482), phospho-p53 at Ser6 (#9285), Ser9 (#9288), Ser15 (#9284), Ser20 (#9287), and Ser37 (#9289), phospho-pRb at Ser807/811 (#9308), mTOR (#2972), Raptor (#2280), Rictor (#2140), TSC2 (#6935), 4EBP1 (#9452), and phospho-4EBP1 at Thr37/46 (#2855) (Cell Signaling Technology, Danvers, MA, USA); PTEN (#SC-7974), MDM2 (#SC-965), phospho-PKCα at Ser657 (#SC-12356), and p21 (#SC-397) (SantaCruz, Dallas, TX, USA); p53 (#NCL-L-p53-DO7) (DO7, Leica, Milton Keynes, UK); Actin (#G043) (Applied Biological Materials Inc.); Flag (#A8592), and Catalase (#C-0979) (Sigma); Myc (#OP10) (Calbiochem, CA, USA).
Jung S.H., Hwang H.J., Kang D., Park H.A., Lee H.C., Jeong D., Lee K., Park H.J., Ko Y.G, & Lee J.S. (2018). mTOR kinase leads to PTEN-loss-induced cellular senescence by phosphorylating p53. Oncogene, 38(10), 1639-1650.
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2

Erlotinib Signaling Pathway Analysis

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Erlotinib (#S1023) was obtained from Selleck Chemicals (Houston, TX, USA). Puromycin (#A1113803) was purchased from Life Technologies (Carlsbad, CA, USA). Hoechst 33342 (#H21492) and propidium iodine (#P1304MP) were acquired from Invitrogen (Waltham, MA, USA). The Rictor (#2114), phospho-tyrosine (#5465), phospho-NDRG1 (T346; #5482), NDRG1 (#9485), phospho-Akt (S473; #4060), Akt (#9272), phospho-S6 (S240/4; #2215), S6 (#2217), phospho-4EBPl (T37/46; #2855), 4EBP1 (#9452), phospho-EGFR (Y1068; #3777), EGFR (#4267) and MET (#8198) antibodies were from Cell Signaling Technologies (Danvers, MA, USA). Antibodies against β-actin (#A1978) were purchased from Sigma (St. Louis, MO, USA).
Chiang C.T., Demetriou A.N., Ung N., Choudhury N., Ghaffarian K., Ruderman D.L, & Mumenthaler S.M. (2018). mTORC2 contributes to the metabolic reprogramming in EGFR tyrosine-kinase inhibitor resistant cells in non-small cell lung cancer. Cancer letters, 434, 152-159.
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3

Western Blot Analysis of CIT, NDRG1, and GAPDH

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Western blot was done using BioRad pre-casted gels and a trans-turbo transfer machine (BioRad). The following are the antibodies used in this study: CIT antibody (BD Biosciences # 611376), NDRG1 (Cell Signaling # 9485), phospho-NDRG1 (Cell Signaling # 5482) and GAPDH (Sana Cruz # SC32233).
Davies R., Liu L., Taotao S., Tuano N., Chaturvedi R., Huang K.K., Itman C., Mandoli A., Qamra A., Hu C., Powell D., Daly R.J., Tan P, & Rosenbluh J. (2021). CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Genome Biology, 22, 47.
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4

In Vitro and In Vivo Compound Preparation

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For in vitro studies MLN0128 (Selleckchem Houston, TX, USA) was dissolved in DMSO, phenfomin was dissolved in DMEM. For in vivo studies MLN0128 was dissolved in 1-methyl-2-pyrrolidinone (NMP), then diluted in 15% PEG diluted in water; phenformin was diluted in water at 1.8 mg/ml. Rapamycin was purchased from LC Laboratories (Woburn, MA) and dissolved in DMSO. Antibodies from Cell Signaling Technology (Beverly, MA, USA) used for immunoblots were diluted 1:1,000 and included phospho-p70 S6 kinase (Thr389 #9234), phospho-S6 (Ser235/236 #4858), total 4E-BP1 (#9644), phospho-4E-BP1 (Thr37/46 #2855), phospho-Akt (Ser473 #4060), phospho-Akt (Thr308 #4056), phospho-NDRG1 (Thr346 #5482), Phospho-Tuberin/TSC2 (Thr1462 #3611), phospho-GSKα/β (Ser21/9 #9331), beta-actin (#4970), cleaved PARP (Asp214 #5625), cleaved caspase 3 (Asp175 #9661) and phospho-Raptor (Ser792 #2083). Anti-Hif-1alpha (C-Term #10006421 1:200) antibody was purchased from Cayman Chemical and anti-GLUT1 (GT11-A 1:1,000) antibody was purchased from Alpha Diagnostic International (San Antonio, TX, USA). Plasmid expressing dox-inducible 4EBP1 4Ala (pCW57.1-4EBP1_4xAla, plasmid # 38240) and control vector (pCW57.1, plasmid # 41393) were purchased from Addgene.
Momcilovic M., McMickle R., Abt E., Seki A., Simko S.A., Magyar C., Stout D.B., Fishbein M.C., Walser T.C., Dubinett S.M, & Shackelford D.B. (2015). Heightening energetic stress selectively targets LKB1-deficient non-small cell lung cancers. Cancer research, 75(22), 4910-4922.
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5

Erlotinib Signaling Pathway Analysis

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Erlotinib (#S1023) was obtained from Selleck Chemicals (Houston, TX, USA). Puromycin (#A1113803) was purchased from Life Technologies (Carlsbad, CA, USA). Hoechst 33342 (#H21492) and propidium iodine (#P1304MP) were acquired from Invitrogen (Waltham, MA, USA). The Rictor (#2114), phospho-tyrosine (#5465), phospho-NDRG1 (T346; #5482), NDRG1 (#9485), phospho-Akt (S473; #4060), Akt (#9272), phospho-S6 (S240/4; #2215), S6 (#2217), phospho-4EBPl (T37/46; #2855), 4EBP1 (#9452), phospho-EGFR (Y1068; #3777), EGFR (#4267) and MET (#8198) antibodies were from Cell Signaling Technologies (Danvers, MA, USA). Antibodies against β-actin (#A1978) were purchased from Sigma (St. Louis, MO, USA).
Chiang C.T., Demetriou A.N., Ung N., Choudhury N., Ghaffarian K., Ruderman D.L, & Mumenthaler S.M. (2018). mTORC2 contributes to the metabolic reprogramming in EGFR tyrosine-kinase inhibitor resistant cells in non-small cell lung cancer. Cancer letters, 434, 152-159.
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