Anti cd154 mab clone mr 1 be0017 1

Full-thickness tail skin was sutured as 1 cm² grafts onto the recipient’s back. Vascularized cardiac allografts were transplanted intra-abdominally using the technique of Corry and colleagues (Corry et al., 1973 (link)). Heart graft rejection was defined as cessation of palpable myocardial contraction, confirmed at explant for histology. In certain experiments, recipients of BALB/c heart allografts were injected i.p. with 500 μg anti-CD154 mAb (clone MR-1; BE0017-1; Bio X Cell) on days −2, 0, 2, and 4 in relation to transplantation, a protocol that prevents acute allograft rejection but that results in development of chronic allograft vasculopathy.

Fully vascularized cardiac allografts were transplanted intra-abdominally (Conlon et al., 2012b (link)). Rejection, defined as cessation of palpable myocardial contraction, was confirmed at explant. Grafts were excised at predetermined time points after transplantation and stored at −80°C or fixed in 10% buffered formalin. In certain experiments, heart allografts were retrieved from donor mice challenged with a recipient strain skin allograft 6 weeks earlier, or recipients were additionally injected intraperitoneally (i.p.) with 500 μg anti-CD154 mAb (clone MR-1; BE0017-1; Bio X Cell) on days −2 and 0 in relation to transplantation, a protocol that prevents acute allograft rejection but that results in development of chronic allograft vasculopathy.