Ta11pa c40 radiotelemetric probes

Similarly as in our previous studies and in accordance with the recommendation for BP measurements in experimental animals, we used radiotelemetry system for direct BP measurements [14 (link), 15 (link), 17 (link), 18 (link), 37 (link), 44 (link), 45 (link)]. At week labeled -4 (i.e. 4 weeks after 5/6 NX or after sham-operation), rats were anesthetized with a combination of tiletamine, zolazepam (Zoletil, Virbac SA, Carros Cedex, France; 8 mg.kg⁻¹), and xylazine (Rometar, Spofa, Czech Republic; 4 mg.kg⁻¹) intramuscularly, and TA11PA-C40 radiotelemetric probes (Data Sciences International, St. Paul, MN, USA) were implanted into abdominal aorta for direct BP measurements as described previously [14 (link), 15 (link)]. Rats were allowed 7 days to recover before basal BP was recorded and only animals with stable BP records at the end of this recovery period were used for experiments.

In accordance with the current recommendations for BP measurement in experimental animals, we employed a radiotelemetry system for direct BP measurements [62 (link)]. Rats were anesthetized with a combination of tiletamine, zolazepam (Zoletil; Virbac SA, Carros Cedex, France; 8 mg/kg), and xylazine (Rometar, Spofa, Czech Republic; 4 mg/kg), given intramuscularly, and TA11PA-C40 radiotelemetric probes (Data Sciences International, St. Paul, Minnesota, USA) were implanted for direct BP measurements as described previously [57 (link),61 (link)]. The rats were allowed 7 days to recover before basal BP was recorded and only animals with stable BP records at the end of this recovery period were used for experiments. Basal BP was determined for 3 days and then the treatment was initiated and continued for 14 days. In the animals implanted with radiotransmitters, 24-h urine samples were collected before the start of pharmacological treatment (day −1), and then on days 1, 6 and 13 of treatment, to assess daily sodium excretion and albuminuria. At the end of experiments, all animals were killed by an overdose of thiopental sodium (Sandoz, Basel, Switzerland) and the ratio of LV weight (LVW) to tibia length was used to evaluate the degree of LVH [48 (link),49 (link),57 (link),61 (link)]. The following groups were examined: