Ti e upright microscope

Manufactured by Nikon

Sourced in Japan

The Ti E upright microscope is a high-performance laboratory equipment designed for a wide range of microscopy applications. It features a sturdy and stable construction, advanced optical systems, and intuitive user interface.

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Lab products found in correlation

Coolsnap hq2 ccd camera, by Nikon (2 mentions) Superfrost plus slide, by Thermo Fisher Scientific (2 mentions) Microtome, by Leica (1 mentions) Dissecting microscope, by Nikon (1 mentions)

Close spelling variants of this product

Ti-E microscope Upright microscope Ti microscope

3 protocols using ti e upright microscope

Immunohistochemical Characterization of Spheroids

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Spheroids were harvested and frozen in Tissue-Tek® O.C.T., (Miles USA, Inc. Elkhart, IN). Eight μm cryostat sections were obtained with a Leica CM1950 cryotome and transferred on to Superfrost™ Plus slides (Fischer Scientific, Boston, MA) for immunostaining. Slides were incubated in polyclonal rabbit anti-human fibronectin (Q0149, Dako) and collagen Type IV mouse monoclonal antibodies for 30 min at room temperature followed by labelling using a Dako Envision + kit (IR630) from Dako North America Inc. (Carpinteria, CA) according to the manufacturer’s instructions. Signal was visualized with 3,3′-Diaminobenzidine (DAB) followed by a light hematoxylin counterstain. Images were taken at 40X magnification using a Nikon Ti E upright microscope with a Cool SNAP HQ2 CCD camera (Tokyo, Japan) and processed with NIS-Elements basic research software.

Jyoti A., Fugit K.D., Sethi P., McGarry R.C., Anderson B.D, & Upreti M. (2015). An in vitro assessment of liposomal topotecan simulating metronomic chemotherapy in combination with radiation in tumor-endothelial spheroids. Scientific Reports, 5, 15236.

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Cryostat H&E Staining Protocol

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Hematoxylin and eosin (H&E) staining was performed by staining the cryostat sections of TES with Harris hematoxylin (aluminum potassium sulfate, hematoxylin, absolute alcohol, mercuric oxide, and glacial acetic acid) followed by 1% acid alcohol and, subsequently, 1% eosin. Images were taken at 40X magnification using a Nikon Ti E upright microscope with a Cool SNAP HQ2 CCD camera (Tokyo, Japan) and processed with NIS-Elements basic research software.

Upreti M., Jyoti A., Johnson S.E., Swindell E.P., Napier D., Sethi P., Chan R., Feddock J.M., Weiss H.L., O'Halloran T.V, & Mark Evers B. (2016). Radiation-enhanced therapeutic targeting of galectin-1 enriched malignant stroma in triple negative breast cancer. Oncotarget, 7(27), 41559-41574.

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Histological Analysis of Rare Mammalian Skin

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Formalin-fixed Choeropsis liberiensis and Hippopotamus amphibius skin samples were first hydrated and rinsed in 1X PBS. Samples were then dehydrated through an ethanol gradient (from 25% to 100%), processed through histoclear and embedded in paraffin. Each hydration and dehydration step lasted for 12 hours. Tissues were sectioned at a thickness of 10 μm with a microtome (Leica). Samples were stained with hematoxylin and eosin using standard methods with minor modifications. Tissue sections were mounted with Permount mounting media and visualized with Nikon Ti-E Upright microscope. Tissue whole-mounts were captured with a Nikon dissecting microscope. Individual fields of Eschrichtius robustus rostral skin were visualized and stitched together with Keyence microscope.

Springer M.S., Guerrero-Juarez C.F., Huelsmann M., Collin M.A., Danil K., McGowen M.R., Won Oh J., Ramos R., Hiller M., Plikus M.V, & Gatesy J. (2021). Genomic and anatomical comparisons of skin support independent adaptation to life in water by cetaceans and hippos. Current biology : CB, 31(10), 2124-2139.e3.

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