Log In Sign up
The largest database of trusted experimental protocols

Anti rock2 antibody

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

The Anti-ROCK2 antibody is a research-use only product designed to detect the ROCK2 protein. ROCK2 is a serine/threonine-protein kinase that plays a role in regulating the organization of the actin cytoskeleton. This antibody can be used for applications such as Western blotting and immunohistochemistry to identify and study the ROCK2 protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Cell Signaling Technology (1 mentions) Genetools software, by Syngene (1 mentions) Anti phospho pkc, by Cell Signaling Technology (1 mentions) Gf109203x, by Merck Group (1 mentions) L name, by Merck Group (1 mentions) Anti pkc, by Cell Signaling Technology (1 mentions) Anti mlc20, by Cell Signaling Technology (1 mentions) Dexmedetomidine, by Merck Group (1 mentions)

3 protocols using anti rock2 antibody

1

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used: anti-ROCK2 antibody (1:1000; Santa Cruz Biotechnology) and anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:8000, Cell Signaling). GeneTools software (version 3.03; Syngene, Cambridge, UK) was used to measure the intensities of the protein bands.
Ye Z., Yin S., Su Z., Bai M., Zhang H., Hei Z, & Cai S. (2016). Downregulation of miR-101 contributes to epithelial-mesenchymal transition in cisplatin resistance of NSCLC cells by targeting ROCK2. Oncotarget, 7(25), 37524-37535.
+ Open protocol
+ Expand
2

ROCK1/2 Kinase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For these assays, which utilize exogenous recombinant MYPT1 as the ROCK substrate (Mong and Wang 2009 (link)), ROCK1 or ROCK2 were first immunoprecipitated from whole cell extracts of purified CD4+ T cells using an anti-ROCK1 or anti-ROCK2 antibody (Santa Cruz Biotechnology). The immunoprecipitated ROCK1 or ROCK2 was then subjected to an in vitro kinase reaction by incubating with purified recombinant MYPT1 substrate added exogenously in kinase buffer containing ATP according to the manufacturer’s instructions (Cell Biolabs). Phosphorylation of recombinant MYPT1 substrate by immunoprecipitated ROCK1 or ROCK2 was detected by Western blotting using an antiphospho-MYPT1 (T696) antibody.
Weng C.H., Gupta S., Geraghty P., Foronjy R, & Pernis A.B. (2016). Cigarette Smoke inhibits ROCK2 activation in T cells and modulates IL-22 production. Molecular immunology, 71, 115-122.
+ Open protocol
+ Expand
3

Reagent Preparation for Signaling Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used pharmaceuticals and chemicals that met the highest purity standards and were readily available in the market. Dexmedetomidine, NaF, GF109203X, L-NAME, and PDBu were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-PKC, anti-phospho-PKC, anti-MLC20, and anti-phopho-MLC20 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Y-27632 was provided by Calbiochem (La Jolla, CA, USA). Anti-ROCK-2 antibody was purchased from Santa Cruz (CA, USA). PDBu and GF109203X were dissolved in dimethyl sulfoxide, with the final concentration of dimethyl sulfoxide being <0.1%. Unless specified otherwise, all medications were dissolved and thinned with distilled water.
Lee S., Hwang Y., Park K.E., Bae S., Ok S.H., Ahn S.H., Sim G., Bae M, & Sohn J.T. (2024). Hypothermia Inhibits Dexmedetomidine-Induced Contractions in Isolated Rat Aortae. International Journal of Molecular Sciences, 25(5), 3017.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!