JZL184 (Cat. no. 13158; ≥97% purity) was purchased from Cayman Chemical. ML239 (SML0442; ≥98%), NSC23766 (SML0952; ≥97%), CP-100356 (PZ0171; ≥98%), elacridar (SML0486; ≥98%), ML210 (SML0521; ≥98%), ferrostatin-1 (SML0583; ≥95%), α-tocopherol (T3251; ≥99%), and N-acetyl-L-cysteine (A7250; ≥99%) were purchased from Sigma. YM-155 (S1130; ≥99%), obatoclax mesylate (S1057; ≥99%), and RITA (S2781; 99%) were purchased from SelleckChem. SC-26196 (4189; 99.2%), quercetin (1125; >98%), and MK-571 (2238; >96.9%) were purchased from Tocris. Austocystin D was purchased from eMolecules. The selective CYP2J2 inhibitor 1-(4-bromophenyl)-4-[4-(hydroxydiphenylmethyl)piperidin-1-yl]butan-1-one was synthesized in-house according to the published methodology (>95% purity)48 (link). All small molecules were dissolved in DMSO, except N-acetylcysteine, which was dissolved in cell culture medium then adjusted to pH 7.5.
Quercetin
Manufactured by Bio-Techne
Sourced in United States
Quercetin is a flavonoid compound found naturally in various plant sources. It is a common laboratory reagent used in biochemical research and analysis. Quercetin can be utilized in a variety of in vitro applications to study its biological properties and interactions.
Automatically generated - may contain errors
Lab products found in correlation
Jzl184, by Cayman Chemical (2 mentions)
α tocopherol, by Merck Group (2 mentions)
Nsc23766, by Merck Group (2 mentions)
Mk571, by Bio-Techne (2 mentions)
Obatoclax mesylate, by Selleck Chemicals (2 mentions)
Ml239, by Merck Group (2 mentions)
Sc 26196, by Bio-Techne (2 mentions)
Cp100356, by Merck Group (2 mentions)
Ym155, by Selleck Chemicals (2 mentions)
N acetyl l cysteine, by Merck Group (2 mentions)
Ferrostatin 1, by Merck Group (2 mentions)
Elacridar, by Merck Group (2 mentions)
Ml210, by Merck Group (2 mentions)
Pifithrin μ, by Merck Group (1 mentions)
Myricetin, by Merck Group (1 mentions)
Infinite m1000, by Tecan (1 mentions)
Kribb11, by Merck Group (1 mentions)
L sulforaphane, by Cayman Chemical (1 mentions)
4 vinylcatechol, by LGC (1 mentions)
Ellagic acid, by Bio-Techne (1 mentions)
5 protocols using quercetin
1
Small Molecule Acquisition and Preparation
2
Small Molecule Acquisition and Preparation
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JZL184 (Cat. no. 13158; ≥97% purity) was purchased from Cayman Chemical. ML239 (SML0442; ≥98%), NSC23766 (SML0952; ≥97%), CP-100356 (PZ0171; ≥98%), elacridar (SML0486; ≥98%), ML210 (SML0521; ≥98%), ferrostatin-1 (SML0583; ≥95%), α-tocopherol (T3251; ≥99%), and N-acetyl-L-cysteine (A7250; ≥99%) were purchased from Sigma. YM-155 (S1130; ≥99%), obatoclax mesylate (S1057; ≥99%), and RITA (S2781; 99%) were purchased from SelleckChem. SC-26196 (4189; 99.2%), quercetin (1125; >98%), and MK-571 (2238; >96.9%) were purchased from Tocris. Austocystin D was purchased from eMolecules. The selective CYP2J2 inhibitor 1-(4-bromophenyl)-4-[4-(hydroxydiphenylmethyl)piperidin-1-yl]butan-1-one was synthesized in-house according to the published methodology (>95% purity)48 (link). All small molecules were dissolved in DMSO, except N-acetylcysteine, which was dissolved in cell culture medium then adjusted to pH 7.5.
3
Evaluation of VACV Inhibitors in A549 and HeLa Cells
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A549 or HeLa cells were seeded in 96-well plates the previous day and infected with modified VACV viruses as specified in the text at an MOI of 1 (fluorescence readout) or 0.1 (viral titer). For drug treatment, compounds were added at specified concentrations prior to virus addition. Inhibitor compounds: Triptolide, Quercetin (Tocris Bioscience), KRIBB11 (EMD Millipore), KRIBB3, Pifithrin-μ, Myricetin (Sigma Aldrich), and Heat Shock Protein Inhibitor I/KNK437 (Santa Cruz Biotechnology, Inc.) For fluorophore assays, cells were fixed at 18 hpi with 4% formaldehyde. Plates were read on a Tecan infinite M1000 for Venus (excitation: 515 nm and emission: 528 nm) and mCherry (excitation: 587 nm and emission: 610 nm). For plaque assays, virus was collected 24 hpi and titered by plaque assay.
4
Stability and Use of Catechol Compounds
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All chemicals in pure form were purchased from Sigma-Aldrich with the exception of L-sulforaphane and luteolin from Cayman Chemical, quercetin from Tocris Bioscience, and 4-vinylcatechol from Toronto Research Chemicals. Because 4-vinylcatechol (also known as 3,4-dihydroxystyrene) contains a vinyl group (see Fig 1 ) it polymerizes in the presence of oxygen, similarly to other styrenes. To prevent polymerization, the supplier (Toronto Research Chemicals), provides this compound under inert atmosphere together with 1% w/w butylated hydroxytoluene. In control experiments, we determined that this proportion of butylated hydroxytoluene had no affect on results. Once a vial of 4-vinylcatechol is opened to room air and suspended in water, the integrity of the stock compound begins to deteriorate, and we found it best to open a new vial for each experiment. Dilute 4-vinylcatechol (30 μM, as used in cell culture experiments) is much more stable because the low concentration disfavors polymerization. In general, catechol and alkyl catechols were prepared as 3mM stock solutions in purified water as vehicle, filter sterilized, and diluted to the final concentration indicated for each experment.
5
Cell culture and drug preparation for NCI/ADR-RES ovarian cancer line
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Cell culture and drug preparation. The doxorubicin-resistant human ovarian cancer cell line, NCI/ADR-RES, was provided by Dr Hwa Jeong Lee (Ewha Womans University, Seoul, Korea). The NCI/ADR-RES cells were cultured in RPMI 1640 (WelGENE, Inc., Daegu, Korea) with 10% fetal bovine serum (Invitrogen; Thermo Fisher Scientific, Inc., Waltham , MA, USA) and 1% antibiotics-antimycotic solution (WelGENE, Inc.) in a humidified atmosphere with 5% CO 2 at 37˚C. The RCM extract was prepared by Hanpoong Pharmaceutical Company (Jeonju, Korea). The RCM was dissolved in 30% ethanol. Ellagic acid and quercetin were purchased from Tocris Biosciences (Boston, MA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Ellagic acid was dissolved in distilled water at 25 mM, and quercetin was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) at 30 mg/ml.
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