C myc gtx103436

Indicated cells were seeded on the slides at the density of 1 × 10⁵ cells per well and incubated overnight. After the incubation, the cells were washed with PBS, fixed with 4% paraformaldehyde solution, permeabilized with 0.1% Triton X, and blocked with bovine serum albumin (BSA) for 1 h. Then cells were incubated with c-Myc (GTX103436) (GeneTex, 1:100) primary antibody at 4 °C overnight. Subsequently, the slides were washed with PBS and treated with secondary antibodies, then counterstained with DAPI, and visualized with a confocal microscopy.

The human breast cancer cell lines MDA-MB-231 and BT549 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Both cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM), and the medium was supplemented with 7% fetal bovine serum (FBS; Corning, New York, NY, USA), 1% glutagro (Corning), and penicillin-streptomycin (Corning). Cells were cultured at 37 °C in a humidified atmosphere of 5% CO₂. Olaparib was purchased from MedChemExpress, the PRMT1 inhibitor C7280948 was purchased from Sigma (St. Louis, MO, USA), and cycloheximide (CHX) was obtained from Sigma. pCMV3-C-FLAG-PRMT1 plasmid (HG11210-CF) was purchased from Sino Biological (Wu-Han, China), and pcDNA-cMyc-HA plasmid was kindly provided by Prof. Cheng Chia-Hsiung (Taipei Medical University). Antibodies against PRMT1 (GTX630187), c-Myc (GTX103436), and β-actin (GTX109639) were purchased from GeneTex (San Antonio, TX, USA).