Acquity uplc class 1 xevo g2q tof mass spectrometer

A Waters Acquity UPLC Class I/Xevo G2Q-Tof mass spectrometer (Milford, MA, USA) was used to obtain the high-resolution ESI-MS (HR-ESI-MS) data. ¹H and ¹³C NMR spectra were acquired on a Bruker Avance III 400 spectrometer spectrometer (Karlsruhe, Germany) with TMS as an internal standard. ¹H-¹H DQF-COSY, NOESY, TOCSY, HSQC and HMBC spectra were recorded using standard Bruker programs spectrometer (Topspin 3.5pl6, Karlsruhe, Germany). Middle pressure liquid chromatography (MPLC) was carried out on a BUCHI apparatus spectrometer (BUCHI LABORTECHNIK AG, Flawil, Switzerland) equipped with a C-605 pump and open chromatographic columns of different specifications (without detector). Thin layer chromatography (TLC) analysis was performed on alumina plates coated with silica gel 60 and silica gel 60 RP-18 F₂₅₄ (Merck) spectrometer (Darmstadt, Germany). Chromatographic separation was performed on self-packed columns with silica gel (Anhui Liangchen Silicon Material Co. Ltd., China) and reversed-phase silica gel (ODS-A 12 nm S-50 μm YMC Co., Kyoto, Japan). AChE (EC 3.1.1.7, from electric eel), BChE (EC 3.1.1.8, from equine serum), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide (ATCI), and butyrylthiocholine iodide (BTCI) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

The chemical reactions were monitored by TLC using commercially available alumina plates coated with silica gel 60 F254 (Merck). Chromatographic separation was performed on self-packed columns with silica gel from Qingdao Haiyang Chemical Group Co., Ltd. (PR China), and MPLC was carried out on a BUCHI apparatus equipped with a C-605 pump. ¹H and ¹³C NMR spectra were recorded on a Bruker Avance III 400 spectrometer with TMS as an internal standard. Coupling constant (J) values were presented in Hz, and spin multiplicities were given as s (singlet), d (doublet), t (triplet), q (quartet), br.s (broad singlet) and m (multiplet). A Waters Acquity UPLC Class I/Xevo G2Q-Tof mass spectrometer was used to obtain the high-resolution ESI-MS (HR-ESI-MS) data.