Photonic bioimaging platform

Manufactured by Zeiss

The Photonic BioImaging platform is a comprehensive imaging solution designed to facilitate advanced biological research. It combines high-resolution optical imaging techniques with powerful analytical capabilities to enable detailed visualization and analysis of biological samples. The platform's core function is to provide researchers with a versatile and efficient tool for studying complex cellular and tissue-level processes.

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Lab products found in correlation

Axio imager 2, by Zeiss (2 mentions) Zen imaging software zen 2.0 blue version, by Zeiss (2 mentions)

2 protocols using photonic bioimaging platform

Autoantigen Binding Assay for Anti-HBs

Cited 1 time

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Binding of human anti-HBs and control monoclonal IgG antibodies (mGO53 and ED38; Meffre et al., 2004 (link); Wardemann et al., 2003 (link)) to HEp-2 cell–expressing autoantigens was analyzed at 50 µg/ml by ELISA (AESKULISA ANA-HEp-2, Aesku.Diagnostics), and indirect immunofluorescence assay (IFA; ANA HEp-2 AeskuSlides, Aesku.Diagnostics) following the manufacturer’s instructions. IFA sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification ×40 with 5,000 ms-acquisition using ZEN imaging software (Zen 2.0 blue version, Zeiss) at the Photonic BioImaging platform (Institut Pasteur).

Hehle V., Beretta M., Bourgine M., Ait-Goughoulte M., Planchais C., Morisse S., Vesin B., Lorin V., Hieu T., Stauffer A., Fiquet O., Dimitrov J.D., Michel M.L., Ungeheuer M.N., Sureau C., Pol S., Di Santo J.P., Strick-Marchand H., Pelletier N, & Mouquet H. (2020). Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers. The Journal of Experimental Medicine, 217(10), e20200840.

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Binding of Anti-HIV-1 Env Antibodies to HEp-2 Cells

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Binding of human anti-HIV-1 Env and control monoclonal IgG antibodies (mGO53 [Wardemann et al., 2003 (link)] and ED38 [Meffre et al., 2004 (link)]) to HEp-2 cell–expressing autoantigens was analyzed at 100 µg/ml by indirect IFA (ANA HEp-2 AeskuSlides; Aesku.Diagnostics) following the manufacturer’s instructions in two independent experiments. IFA sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification 40× with 5,000 ms–acquisition using ZEN imaging software (Zen 2.0 blue version; Zeiss) at the Photonic BioImaging platform (Institut Pasteur).

Lorin V., Fernández I., Masse-Ranson G., Bouvin-Pley M., Molinos-Albert L.M., Planchais C., Hieu T., Péhau-Arnaudet G., Hrebík D., Girelli-Zubani G., Fiquet O., Guivel-Benhassine F., Sanders R.W., Walker B.D., Schwartz O., Scheid J.F., Dimitrov J.D., Plevka P., Braibant M., Seaman M.S., Bontems F., Di Santo J.P., Rey F.A, & Mouquet H. (2022). Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller. The Journal of Experimental Medicine, 219(3), e20212045.

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