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Erk1 2 k 23

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ERK1/2 (K-23) is a protein-specific antibody that recognizes the extracellular signal-regulated kinase 1/2 (ERK1/2) proteins. ERK1/2 are members of the mitogen-activated protein kinase (MAPK) family and play a crucial role in cellular processes such as proliferation, differentiation, and survival.

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Lab products found in correlation

P erk1 2, by Cell Signaling Technology (2 mentions) Jnk c 17, by Cell Signaling Technology (2 mentions) Ran c 20, by Santa Cruz Biotechnology (2 mentions) C ets 1 antibodies c 20, by Santa Cruz Biotechnology (2 mentions) H3k4me3 cs200580 and total h3 antibodies 04 928, by Merck Group (2 mentions) Set1 antibody a300 289a, by Fortis Life Sciences (2 mentions) Traf6 h 274, by Santa Cruz Biotechnology (2 mentions) Stat3 c 20, by Santa Cruz Biotechnology (2 mentions) C myc 9e10, by Santa Cruz Biotechnology (2 mentions) Il 6 blocking antibody, by R&D Systems (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Anti ubiquitin, by Santa Cruz Biotechnology (2 mentions) P jnk, by Cell Signaling Technology (2 mentions) Angiotensin 2, by Merck Group (1 mentions) Pcna pc10, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d, by Wanlei (1 mentions) Anti cdk4, by Wanlei (1 mentions) Anti cdk2, by Wanlei (1 mentions) Anti cycline, by Wanlei (1 mentions) Anti akt and anti phospho akt ser473 antibodies, by Cell Signaling Technology (1 mentions)

3 protocols using erk1 2 k 23

1

Klotho Modulates Angiotensin II-Induced Vascular Remodeling

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Dulbecco’s modified Eagle’s medium (DMEM) was purchased from HyClone (HyClone, Logan, CT, USA). Fetal bovine serum (FBS) was purchased from ABGENT (San Diego, CA, USA). We purchased penicillin, streptomycin, and Angiotensin II from Sigma-Aldrich (St. Louis, MO, USA). The Cell Counting Kit-8 (CCK8) was purchased from Dojindo (Kumamoto, Japan). The rat recombinant Klotho protein was from Cloud-Clone Corporation (Houston, TX, USA). The antibodies used in this study were as follows: mouse monoclonal PCNA (PC10), rabbit polyclonal NF-κB p65 (C-20), rabbit polyclonal p-NF-κB p65 (Ser 536), rabbit polyclonal ERK1/2 (K-23), rabbit polyclonal p-ERK1/2 (Thr202/Tyr204), rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (FL-335) (Santa Cruz Biotechnology, CA, USA); and rabbit polyclonal SM22α (Cloud-Clone Corp., Wuhan, China). Anti-CDK2, anti-CDK4, anti-cyclin D, and anti-cyclin E antibodies were purchased from Wanleibio, Shenyang, China, and anti-Akt and anti-phospho-Akt (Ser473) antibodies were from Cell Signaling (MA, USA). The reactivity with rat antigens is included in all of these antibodies. Through ImageJ software (NIH, USA), the signal intensity was quantified.
Yu S., Chen Y., Chen S., Ye N., Li Y, & Sun Y. (2018). Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal Regulated Kinase (ERK) Signaling Activities. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 4851-4860.
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2

Immunoblotting and ChIP Assays for Cell Signaling Analysis

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The following antibodies were used at the indicated dilutions for immunoblotting: pJNK (#9251, 1:1000), p-p38 (#9211, 1:1000), pERK1/2 (#9101, 1:1000) and pSTAT3 antibodies (#9131, 1:1000), obtained from Cell Signaling; JNK (C-17, 1:1000), p38 (H-147, 1:1000), ERK1/2 (K-23, 1:1000), TRAF6 (H-274, 1:1000), anti-ubiquitin (P4D1, 1:1000), RAN (C-20, 1:1000), STAT3 (C-20, 1:1000), c-Myc (9E10, 1:5,000) and tubulin antibodies (10D8, 1:2000), obtained from Santa Cruz Biotech; and anti-K63 ubiquitin (HWA4C4, 1:750) and anti-Ubc13 antibodies (#371100, 1:1000), obtained from Enzo and Invitrogen, respectively. TRAF6 antibody (H-274) was also used for immunoprecipitation (1 g/sample). For ChIPs, the following antibodies were used at 2 g/sample: STAT3 (C-20) and c-Ets-1 antibodies (C-20), obtained from Santa Cruz Biotech; H3K4me3 (CS200580) and total H3 antibodies (04-928), obtained from Millipore; and Set1 antibody (A300-289A) from Bethyl Laboratories. For flow cytometry, fluorescently labeled antibodies to CD11b (M1/70, FITC-labeled), F4/80 (BM8, PerCP Cy5.5-labeled), M-CSFR (AFS98, APC-labeled) and RANK (R12-31, PE-labeled) from eBioscience were used at a 1:100 dilution. Recombinant murine RANKL, IL-6, IL-10 and IL-6 blocking antibody were purchased from R&D Systems.
Zhang H., Hu H., Greeley N., Jin J., Matthews A.J., Ohashi E., Caetano M.S., Li H.S., Wu X., Mandal P.K., McMurray J.S., Moghaddam S.J., Sun S.C, & Watowich S.S. (2014). STAT3 restrains RANK- and TLR4-mediated signaling by suppressing expression of the E2 ubiquitin ligase Ubc13. Nature communications, 5, 5798.
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3

Immunoblotting and ChIP Assays for Cell Signaling Analysis

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The following antibodies were used at the indicated dilutions for immunoblotting: pJNK (#9251, 1:1000), p-p38 (#9211, 1:1000), pERK1/2 (#9101, 1:1000) and pSTAT3 antibodies (#9131, 1:1000), obtained from Cell Signaling; JNK (C-17, 1:1000), p38 (H-147, 1:1000), ERK1/2 (K-23, 1:1000), TRAF6 (H-274, 1:1000), anti-ubiquitin (P4D1, 1:1000), RAN (C-20, 1:1000), STAT3 (C-20, 1:1000), c-Myc (9E10, 1:5,000) and tubulin antibodies (10D8, 1:2000), obtained from Santa Cruz Biotech; and anti-K63 ubiquitin (HWA4C4, 1:750) and anti-Ubc13 antibodies (#371100, 1:1000), obtained from Enzo and Invitrogen, respectively. TRAF6 antibody (H-274) was also used for immunoprecipitation (1 g/sample). For ChIPs, the following antibodies were used at 2 g/sample: STAT3 (C-20) and c-Ets-1 antibodies (C-20), obtained from Santa Cruz Biotech; H3K4me3 (CS200580) and total H3 antibodies (04-928), obtained from Millipore; and Set1 antibody (A300-289A) from Bethyl Laboratories. For flow cytometry, fluorescently labeled antibodies to CD11b (M1/70, FITC-labeled), F4/80 (BM8, PerCP Cy5.5-labeled), M-CSFR (AFS98, APC-labeled) and RANK (R12-31, PE-labeled) from eBioscience were used at a 1:100 dilution. Recombinant murine RANKL, IL-6, IL-10 and IL-6 blocking antibody were purchased from R&D Systems.
Zhang H., Hu H., Greeley N., Jin J., Matthews A.J., Ohashi E., Caetano M.S., Li H.S., Wu X., Mandal P.K., McMurray J.S., Moghaddam S.J., Sun S.C, & Watowich S.S. (2014). STAT3 restrains RANK- and TLR4-mediated signaling by suppressing expression of the E2 ubiquitin ligase Ubc13. Nature communications, 5, 5798.
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