All reagents in this study were commercial products used without further purification. Sodium [125I]iodide ([125I]NaI) and [123I]NaI were purchased from PerkinElmer and FUJIFILM RI Pharma Co., Ltd., respectively. Smart Flash EPCLC W-Prep 2XY (Yamazen Corporation) was used for silica gel chromatography. 1H NMR was recorded on a JNM-ECS400 (JEOL) with tetramethylsilane (TMS) as an internal standard. Coupling constants are reported in Hertz (Hz). Multiplicity was defined as singlet (s), doublet (d), triplet (t), multiplet (m), and quartet (q). ESI mass spectrometry was conducted with a Shimadzu LCMS-2020. High-resolution mass spectrometry (HRMS) was conducted with JMS-700V (JEOL). High-performance liquid chromatography (HPLC) was performed with Shimadzu system (an LC-20AD pump with an SPD-20A UV detector, λ = 254 nm) using a Cosmosil C18 column (Nacalai Tesque, COSMOSIL 5C18-AR-II 4.6 mm I.D. × 150 mm) and CH3CN/H2O (60: 40) as the mobile phase at a flow rate of 1.0 mL/min. HER2-expressing human gastric cancer cells (N87) were purchased from DS Pharma Biomedical (Osaka, Japan).
Jms 700v
Manufactured by JEOL
Sourced in Japan
The JMS-700V is a high-performance mass spectrometer designed for chemical analysis. It provides accurate and precise measurement of molecular weights and chemical compositions of various samples.
Automatically generated - may contain errors
Lab products found in correlation
Cosmosil c18 column, by Nacalai Tesque (1 mentions)
123i nai, by PerkinElmer (1 mentions)
Lcms 2020, by Shimadzu (1 mentions)
Jnm ecs400, by JEOL (1 mentions)
Silica gel 60, by Kanto Chemical (1 mentions)
600 mhz nmr spectrometer, by Agilent Technologies (1 mentions)
Aβ1 42, by Fujifilm (1 mentions)
Tlc silica gel 60 f254, by Merck Group (1 mentions)
Jnm al400, by JEOL (1 mentions)
Catechin, by Merck Group (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
P 2200, by Jasco (1 mentions)
Genequant 1300, by GE Healthcare (1 mentions)
3500 genetic analyzer, by Thermo Fisher Scientific (1 mentions)
Jnm eca500, by JEOL (1 mentions)
Ft ir 4100, by Jasco (1 mentions)
4 protocols using jms 700v
1
Radiolabeled Iodine Compounds Synthesis and Analysis
2
Amyloid-beta Peptide Preparation Protocol
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Unless otherwise specified, all commercially
available compounds were used as provided without further purification.
Solvents for chromatography were of technical grade. Reactions were
monitored by thin-layer chromatography (TLC) carried out on silica
gel plates (TLC Silica Gel 60 F254, Merck). Column chromatography
was performed using Silica Gel 60 (spherical, 63–210 μm,
KANTO CHEMICAL). 1H and 13C nuclear magnetic
resonance (NMR) spectra were recorded with a JEOL JNMAL-400 (400 MHz)
NMR and a Varian 600 MHz NMR spectrometer, respectively, using acetone-d6 or dimethyl sulfoxide (DMSO)-d6 as the solvent and internal reference. Mass spectra
were measured with a JMS-700V (JEOL) mass spectrometer. Sib, the mixture
of silybin A and silybin B, was purchased from Sigma Aldrich, Inc.
Th-T and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were purchased from
Sigma-Aldrich, Inc. Aβ1–42 (hydrochloride
salt) was purchased from Wako Pure Chemical Industries, Ltd. Aβ1–42 (trifluoroacetic acid salt) was purchased from
Peptide Institute, Inc. Prior to experiments, Aβ1–42 was completely dissolved in HFIP using sonication. After dispensing
the solution into appropriate volumes, the solvent was evaporated
and the resultant Aβ1–42 was stored at −80
°C before use. Reagents for cell culture were used as provided
without further purification.
available compounds were used as provided without further purification.
Solvents for chromatography were of technical grade. Reactions were
monitored by thin-layer chromatography (TLC) carried out on silica
gel plates (TLC Silica Gel 60 F254, Merck). Column chromatography
was performed using Silica Gel 60 (spherical, 63–210 μm,
KANTO CHEMICAL). 1H and 13C nuclear magnetic
resonance (NMR) spectra were recorded with a JEOL JNMAL-400 (400 MHz)
NMR and a Varian 600 MHz NMR spectrometer, respectively, using acetone-d6 or dimethyl sulfoxide (DMSO)-d6 as the solvent and internal reference. Mass spectra
were measured with a JMS-700V (JEOL) mass spectrometer. Sib, the mixture
of silybin A and silybin B, was purchased from Sigma Aldrich, Inc.
Th-T and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were purchased from
Sigma-Aldrich, Inc. Aβ1–42 (hydrochloride
salt) was purchased from Wako Pure Chemical Industries, Ltd. Aβ1–42 (trifluoroacetic acid salt) was purchased from
Peptide Institute, Inc. Prior to experiments, Aβ1–42 was completely dissolved in HFIP using sonication. After dispensing
the solution into appropriate volumes, the solvent was evaporated
and the resultant Aβ1–42 was stored at −80
°C before use. Reagents for cell culture were used as provided
without further purification.
3
Purification and Characterization of (+)-Catechin
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The reagents and solvents were of commercial origin (Wako Chemicals, Tokyo, Japan; Tokyo Chemical Industry, Tokyo, Japan; Sigma-Aldrich, St. Louis, MO, USA) and were used without further purification. (+)-Catechin (Sigma-Aldrich) was dried under vacuum for 12 h. All reactions were monitored by thin-layer chromatography on silica gel 60 F254 (0.25 nm, Merck KGaA, Darmstad, Germany) using UV light and a sulfuric acid–phosphomolybdic acid solution in ethanol for detection. Column chromatography was performed using silica gel 60 (0.063–0.200 mm, Merck) unless otherwise stated. 1H and 13C NMR spectra were recorded with a JEOL JNMAL-400 (400 MHz) NMR spectrometer using CDCl3, DMSO-d6, or CD3OD as the solvent and tetramethylsilane as the internal reference. Mass spectra were measured using a JMS-700V (JEOL) mass spectrometer. The purity of all compounds was determined to be >95% using 1H and 13C NMR spectroscopy.
4
Maniwamycin C-F Production from Soil Isolate
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Bacterial strain, TOHO-M025, was isolated from a soil sample collected in Iwaki, Fukushima, Japan, and was used for the production of maniwamycins C-F. C. violaceum CV026, 15 used to evaluate quorum-sensing inhibition activity, was kindly provided by Dr T. Ikeda (Utsunomiya University, Japan).
UV spectra were recorded on a spectrophotometer (GeneQuant1300, GE Healthcare Life Sciences, Little Chalfont, UK). IR spectra were recorded on a Fourier transform IR spectrometer (FT/IR-4100, JASCO, Tokyo, Japan). Optical rotations were measured with a digital polarimeter (P-2200, JASCO). FAB-MS and HR FAB-MS spectra were recorded using a mass spectrometer (JMS-700 V, JEOL, Tokyo, Japan). The various NMR spectra were also determined with a spectrometer (JNM-ECA500, JEOL). 16S ribosomal RNA fragments of TOHO-M025 was amplified by PCR using the general bacterial 16S ribosomal RNA primers, 10F (5′-AGTTTGATCCTGGCTC-3′) and 1100R (5′-CAGGAAGGGTTGCGCT-3′). The DNA sequences of the amplified fragments were determined by cycle sequencing with the chain termination technique and dye-labeled dideoxynucleotides using a Genetic Analyzer 3500 (Applied Biosystems, Foster City, CA, USA).
UV spectra were recorded on a spectrophotometer (GeneQuant1300, GE Healthcare Life Sciences, Little Chalfont, UK). IR spectra were recorded on a Fourier transform IR spectrometer (FT/IR-4100, JASCO, Tokyo, Japan). Optical rotations were measured with a digital polarimeter (P-2200, JASCO). FAB-MS and HR FAB-MS spectra were recorded using a mass spectrometer (JMS-700 V, JEOL, Tokyo, Japan). The various NMR spectra were also determined with a spectrometer (JNM-ECA500, JEOL). 16S ribosomal RNA fragments of TOHO-M025 was amplified by PCR using the general bacterial 16S ribosomal RNA primers, 10F (5′-AGTTTGATCCTGGCTC-3′) and 1100R (5′-CAGGAAGGGTTGCGCT-3′). The DNA sequences of the amplified fragments were determined by cycle sequencing with the chain termination technique and dye-labeled dideoxynucleotides using a Genetic Analyzer 3500 (Applied Biosystems, Foster City, CA, USA).
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