LHPP siRNA (human LHPP ON-TARGET plus set of 4, pooled, catalogue number LQ-018950-02-0005; siRNA 1 CAACCCAAACUGUGUGGUA, siRNA 2 CAUGAAGGCGCUUGAGUAU, siRNA 3 GCAGCACGCCGACAAGUGA, siRNA 4 CUGAAGCGUUCCCG GCUGA) and negative control siRNA (ON-TARGET plus non-targeting pool) were purchased from Dharmacon. SNU449 cells were transfected in the presence of the transfection reagent jetPRIME (PolyPlus transfection) as per the manufacturer’s instructions. Cells were then re-suspended in complete DMEM plus 10% FBS medium. Then 36 h after transfection, SNU449 cells were re-transfected with LHPP siRNA and were seeded in a 6-well plate (4 × 104 per well). The cells were stained with crystal violet (2% crystal violet in 20% methanol) on day 3.
Sirna 4
Manufactured by Horizon Discovery
SiRNA 4 is a laboratory equipment used for gene silencing. It utilizes small interfering RNA (siRNA) molecules to inhibit the expression of target genes, enabling researchers to study gene function and explore potential therapeutic applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sirna 4
1
LHPP siRNA Knockdown Assay
2
Transfection of Cell Lines with siRNA
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Transfections were performed as previously described [43 (link)], using either Oligofectamine or Lipofectamine 2000. PMAIP1 siRNA and BAK siRNA were purchased from Ambion. The two Bcl-xL and two c-myc specific siRNAs were purchased from CST: Cell Signaling Technology, Danvers, MA. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05), ATF4 (SMARTpool: ON-TARGETplus ATF4 siRNA, L-005125-00-0005) and four individual Mcl-1 siRNAs (ON-TARGETplus Mcl-1 siRNAs; siRNA-1: GGU UUG GCA UAU CUA AUA A; siRNA-2: GAA GGU GGC AUC AGG AAU G, siRNA-3: GAU UAU CUC UCU CGG UAC CUU, siRNA-4: CGA AGG AAG UAU CGA AUU U (Dharmacon)) were purchased from Thermo Fisher Scientific.
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