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Dmem f12 with hepes dmem f12 hepes

Manufactured by Thermo Fisher Scientific

DMEM-F12 with HEPES (DMEM-F12/HEPES) is a cell culture medium formulation that provides nutrients and a buffering system to support the growth and maintenance of various cell lines. The medium is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture, supplemented with the buffer HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) to help maintain the desired pH level during cell culture.

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2 protocols using dmem f12 with hepes dmem f12 hepes

1

Primary Cell Culture Reagents

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Hanks Balanced Saline solutions (HBSS), Dulbecco's Modified Eagle Medium—Hams'F12 nutrient mixture (DMEM-F12), DMEM-F12 with HEPES (DMEM-F12/HEPES), 0.25% trypsin-EDTA, fetal bovine serum (FBS), and Penicillin-Streptomycin (P/S) were from Gibco (ThermoFisher Scientific, Burlington, ON). ATP, glutamate, lidocaine HCl, Triton X-100, LPS, and sodium nitrite standard solution were from Sigma (Oakville, ON).
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2

Cell Culture Reagents for Experiments

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Hanks Balanced Saline solutions (HBSS), Dulbecco's Modified Eagle Medium -Hams'F12 nutrient mixture (DMEM-F12), DMEM-F12 with HEPES (DMEM-F12/HEPES), 0.25% trypsin-EDTA, fetal bovine serum (FBS), and Penicillin-Streptomycin (P/S) were from Gibco (ThermoFisher Scientific, Burlington, ON). ATP, glutamate, lidocaine HCl, Triton X-100, LPS, and sodium nitrite standard solution were from Sigma (Oakville, ON).
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