Uv tbx pmt series detector

Fluorescence spectroscopy was employed to gather qualitative insights into the internal structure and microenvironment of the prepared systems in HPLC-grade water. Pyrene was used as a hydrophobic probe, capable of being incorporated into the hydrophobic domains of the hybrid platforms. A NanoLog Fluorometer (Horiba Jobin Yvon, Piscataway, NJ, USA) was used to record the pyrene emission spectra, with a laser diode serving as the excitation source (Nano LED, 440 nm, 100 ps pulse width) and a UV TBX-PMT series detector (250–850 nm) from Horiba Jobin Yvon (Piscataway, NJ, USA). A description of the utilized method is presented below.
Colloidal dispersions of P407, P407/Tw80, P407/Tw80/MβCD, and P407/Tw80/HPβCD were prepared at concentrations of 10 mg/mL. Following this, 3 μL of a pyrene stock solution (1 mM) was added to each colloidal dispersion. The dispersions were equilibrated for 24 h and the I₁/I₃ ratio was measured at each hybrid system concentration at temperatures of 25 °C, 37 °C, and 50 °C. Fluorescence spectra were collected in the range of 355–630 nm, with an excitation wavelength of 335 nm. Notably, no excimer formation was observed for the examined solutions.

The pyrene fluorescence spectra in the presence of terpolymers and under different solution conditions were recorded with a NanoLog Fluorimeter (Horiba Jobin Yvon, Kyoto, Japan), using a laser diode as the excitation source (NanoLED, 440 nm, pulse width 100 ps) and a UV TBX-PMT series detector (250–850 nm) by Horiba Jobin Yvon. Solutions of each terpolymer were prepared at constant polymer concentration. Pyrene solution in acetone at a ratio of 1 µL/mL was added to each vial. The samples were kept at rest for 24 h to ensure encapsulation of pyrene into the hydrophobic domains of the polymer aggregates and evaporation of acetone. The excitation wavelength used for the measurements was 335 nm. Emission spectra were recorded in the spectral range of 355–640 nm. The ratio I₁/I₃, i.e., the ratio of intensities of the first and third vibronic peaks in pyrene fluorescence spectra, was utilized in order to access the hydrophobicity of pyrene environment.