Four-hundred cells were sorted directly into aliquots of 100 µL lysis buffer (Invitrogen Dynabeads mRNA Direct Kit; Thermo Fisher). Poly(A)-plus RNA was isolated using Invitrogen Dynabeads mRNA Direct Kit (Thermo Fisher, 61012) and sequencing libraries were prepared following Smart-Seq2 optimized protocol (Picelli et al. 2014 (link)). cDNA was quantified with D5000HS TapeStation (Agilent) and final tagmented libraries were quantified on D1000HS TapeStation (Agilent). Libraries were sequenced on Illumina NextSeq500 with 75 cycle High Output Kit v2.5.
Invitrogen dynabeads mrna direct kit
Manufactured by Thermo Fisher Scientific
The Invitrogen Dynabeads mRNA Direct Kit is a magnetic bead-based system designed for the isolation and purification of poly(A) mRNA from various sample types, including cells and tissues. The kit utilizes oligo(dT) coated magnetic beads to selectively capture and separate mRNA molecules, allowing for their subsequent use in downstream applications such as RT-PCR, cDNA synthesis, and gene expression analysis.
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Lab products found in correlation
1 protocol using invitrogen dynabeads mrna direct kit
1
Single-cell RNA-seq using Smart-Seq2
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