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Fast sybr green master mix

Manufactured by Meridian Bioscience
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Sourced in United Kingdom

Fast SYBR Green Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) assays. It contains SYBR Green I dye, a DNA-binding agent that emits fluorescence upon binding to double-stranded DNA, as well as all the necessary components for efficient DNA amplification and detection.

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Lab products found in correlation

Random hexamer, by Thermo Fisher Scientific (1 mentions) Reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Cfx96, by Bio-Rad (1 mentions) Nucleospin rna extraction kit, by Macherey-Nagel (1 mentions) Mirneasy kit, by Qiagen (1 mentions) Nanodrop 1000, by Thermo Fisher Scientific (1 mentions) Sensifast kit, by Meridian Bioscience (1 mentions) Qbase 2, by CellCarta (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SYBR Green (57 citations) SYBR Green mix (12 citations) SYBR Master mix (3 citations)

2 protocols using fast sybr green master mix

1

Total RNA Extraction and qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from the cells using Trizol (Invitrogen) according to the manufacturer’s instructions. The RNA was reverse-transcribed using reverse transcriptase (Invitrogen) and random hexamers (Invitrogen). qPCR was performed in triplicate with Fast SYBR Green Master Mix (Bioline, London, UK) using CFX96 (Bio-Rad, CA, USA). The primers used for qPCR are provided in Supplementary Table S1.
Oh Y., Park R., Kim S.Y., Park S.H., Jo S., Kim T.H, & Ji J.D. (2021). B7–H3 regulates osteoclast differentiation via type I interferon-dependent IDO induction. Cell Death & Disease, 12(11), 971.
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2

RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells resuspended in QIAzol using an miRNeasy Kit and processed according to the manufacturer's instructions (QIAGEN) or in RA1 lysis buffer using the RNA NucleoSpin extraction kit (Macherey&Nagel). RNA was quantified using a NanoDrop 1000 (Thermo Scientific) and 500-2,000 ng was reverse transcribed with a High-Capacity cDNA Reverse Transcription Kit (Life Technologies). qPCRs were run using Fast SYBR Green Master Mix or SensiFast kit (Bioline) and a Roche LightCycler 384 (both from Life Technologies). Data processing with qbase+ 2.6 software (Biogazelle) relies on normalization with a minimum of 2 reference genes. RT-qPCR primer sequences are available upon request.
Rambow F., Rogiers A., Marin-Bejar O., Aibar S., Femel J., Dewaele M., Karras P., Brown D., Chang Y.H., Debiec-Rychter M., Adriaens C., Radaelli E., Wolter P., Bechter O., Dummer R., Levesque M., Piris A., Frederick D.T., Boland G., Flaherty K.T., van den Oord J., Voet T., Aerts S., Lund A.W, & Marine J.C. (2018). Toward Minimal Residual Disease-Directed Therapy in Melanoma. Cell, 174(4).
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