N‐hydroxysuccinimide (NHS)‐activated sepharose beads (GE Healthcare, Uppsala, Sweden) were coated with pooled human serum IgA (300 μg/mL, Sigma‐Aldrich) according to the manufacturer's instructions.
Human serum iga
Manufactured by Merck Group
Sourced in Sweden, United States
Human serum IgA is a laboratory product that is used for the detection and quantification of immunoglobulin A (IgA) in human serum samples. IgA is an antibody class that plays a crucial role in mucosal immunity and immune response.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human serum iga
1
IgA Immobilization on NHS-Sepharose
2
Fluorescent Protein Labeling for Enterococcus
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A DNA-binding fluorescent dye, SYTOX Green (green) from the set SelectFX (Invitrogen, USA), was used for the coloring of E. faecium L3.
Immunohistochemical detection of protein Bac in Enterococcal clones was carried out with human serum IgA (Sigma) conjugated with horseradish peroxidase (HRP). E.faecium L3-Bac+ was incubated with IgA-HRP conjugate for 30 minutes at 25°C. In order to carry out further fluorescence imaging in a confocal laser microscope, a peroxidase was detected by reaction with goat anti-HRP IgG, conjugated with the fluorochrome Cy3 (red) (Jackson ImmunoResearch, USA). The obtained slides were analyzed by confocal argon laser microscope LSM 710 (Zeiss, Germany) (488 nm) or solid state (561 nm) lasers.
Immunohistochemical detection of protein Bac in Enterococcal clones was carried out with human serum IgA (Sigma) conjugated with horseradish peroxidase (HRP). E.faecium L3-Bac+ was incubated with IgA-HRP conjugate for 30 minutes at 25°C. In order to carry out further fluorescence imaging in a confocal laser microscope, a peroxidase was detected by reaction with goat anti-HRP IgG, conjugated with the fluorochrome Cy3 (red) (Jackson ImmunoResearch, USA). The obtained slides were analyzed by confocal argon laser microscope LSM 710 (Zeiss, Germany) (488 nm) or solid state (561 nm) lasers.
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