Example 5
Normal human neonatal foreskin skin fibroblasts (NHDF, KURABO INDUSTRIES LTD.) in the logarithmic growth phase (1440×104 cells) were prepared, suspended in the medium composition (48 mL) of Example C371 in Table 3, dispensed by 30×104 cells (1 mL) to a 24-well cell culture plate (manufactured by SUMITOMO BAKELITE CO., LTD.), and cultured under 37° C., 5% carbon dioxide gas conditions for 3 days. After culturing, the cell concentration of the cell suspension was measured by a cell counter (TC-20, BIO-RAD), the suspension was transferred to a 1.5 mL microtube and pipetted a given number of times using a micropipette (manufactured by Thermo Scientific, clip chip 1000 μL) set to suction/discharge volume 0.2 mL. Thereafter, centrifugation (300×g, 3 min) was performed and the supernatant (1.1 mL) was removed. The cells were resuspended by adding 10% fetal bovine serum-containing DMEM-LG (0.9 mL), and the amount of ATP contained in the cells was quantified by a plate reader (manufactured by Tecan Japan Co., Ltd.) and using CellTiter-Glo (Promega Corporation), and the cell recovery rate was calculated. The above test was performed 5 times each, and the mean thereof is shown in the Table.