Airway epithelium cultured on 6.5-mm Transwell membranes was infected with RSV-GFP (MOI=4) at the apical side (described above). At 5 DPI, live-cell SiR-actin (Cytoskeleton, Inc.) was added to the complete medium at the basal side, and the epithelium was cultured for six hours before imaging using a 20x objective under a Leica DMi8 inverted fluorescence microscope equipped with an environmentally controlled chamber to maintain 37°C and 5% CO2. Images were taken every 5 min to capture the epithelial tissue (phase-contrast), RSV-GFP (AF488), and F-actin (Cy5) for approximately 16 hours. LASX software (Leica Microsystems) installed in the microscope was used to create a movie file.
Live cell sir actin
Manufactured by Cytoskeleton
Live-cell SiR-actin is a fluorescent dye that specifically labels actin filaments in living cells. It enables real-time visualization and analysis of actin dynamics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using live cell sir actin
1
Visualizing RSV Infection in Airway Epithelium
2
Dynamic Imaging of RSV-Infected Airway Epithelium
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Airway epithelium cultured on 6.5 mm Transwell membranes was infected with RSV-GFP (MOI=4) at the apical side (described above). At 5 DPI, live-cell SiR-actin (Cytoskeleton, Inc.) was added to the complete medium at the basal side, and the epithelium was cultured for six hours before imaging using a 20x objective under a Leica DMi8 inverted fluorescence microscope equipped with an environmentally controlled chamber to maintain 37 °C and 5% CO2. Images were taken every 5 min to capture the epithelial tissue (phase-contrast), RSV-GFP (AF488), and F-actin (Cy5) for approximately 16 h. LASX software (Leica Microsystems) installed in the microscope was used to create a movie file.
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