Cells were detached with Accutase (Sigma-Aldrich) and washed two times with PBS supplemented with 2% FCS and 5 mM EDTA. The monoclonal α-Gal antibody clone M86 (EnzoLife Sciences, Inc.) and the secondary anti-mouse IgM-APC (BD Pharmingen) were used to detect α-Gal expression. Samples were measured using a FACSCanto II (Becton Dickinson) flow cytometer and analyzed using FlowJo software (FlowJo).
Anti mouse igm apc
Manufactured by BD
Anti-mouse IgM-APC is a laboratory reagent used for the detection and quantification of mouse immunoglobulin M (IgM) in biological samples. It is conjugated with the fluorescent dye allophycocyanin (APC), which allows for the identification and enumeration of IgM-positive cells through flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (1 mentions)
Accutase, by Merck Group (1 mentions)
Cd19 pe, by BioLegend (1 mentions)
Il 10 pe, by BD (1 mentions)
B220 apc cy7, by BD (1 mentions)
Cd1d bv421, by BD (1 mentions)
Cd43 pe cy7, by BD (1 mentions)
Cd21 apc cy7, by BioLegend (1 mentions)
Cytofix cytoperm kit, by BD (1 mentions)
Cd5 pe cy7, by BioLegend (1 mentions)
Cd5 percp, by BD (1 mentions)
B220 fitc, by BD (1 mentions)
Cd93 pe cy7, by BioLegend (1 mentions)
Fc blocker cd16 cd32, by BD (1 mentions)
2 protocols using anti mouse igm apc
1
Detecting Alpha-Gal Expression by Flow Cytometry
2
Multicolor Flow Cytometry of Immune Cells
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The following antibodies were used for staining: anti-mouse IgM-APC, B220-FITC, CD93-PE CF594, CD19-Alexa Fluor 647, IL10-PE, CD23-BB515, CD5-PerCP, CD1d-BV421, CD43-PE Cy7, B220-APC Cy7, Fc Blocker (CD16/CD32), Cytofix/CytoPerm kit (all from BD Pharmingen), CD23-PerCP/Cy5.5, CD93-PE Cy7, CD1d-PB, CD5-PE Cy7, CD21-APC Cy7, CD19-PE, CD43-APC Cy7 (all from Bio Legend), Live/Dead aqua marker (Molecular Probes).
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