Short-term or single timepoint imaging was performed using either Nikon Plan Apo λ 10x/0.45 or 20x/0.75 objectives with a 0.7x demagnifier and Nikon Eclipse Ti microscope with a Flir, Chameleon3 CMOS camera. Long-term imaging was performed using a Carl Zeiss Plan-Apochromat 10x/0.45 objective with a 0.63x demagnifier and Carl Zeiss Axiovert 200 M microscope also with a Flir, Chameleon3 camera housed inside an incubator for stable environmental control. All imaging was accomplished using custom automated software written using MATLAB and Micro-Manager. For all time-lapse microscopy experiments, the time intervals between image captures were always either 15 or 20 min. Image acquisition software is available on the GitHub repository: https://github.com/wollmanlab/Scope .
Axiovert 200 m microscope
Manufactured by Teledyne
The Axiovert 200 M is an inverted microscope designed for a range of laboratory applications. It features a sturdy, ergonomic design and advanced optics to provide high-quality imaging and analysis capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using axiovert 200 m microscope
1
Automated Time-lapse Microscopy Protocol
2
Automated Single-Cell Virus Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Short-term or single time-point imaging was performed using either Nikon Plan Apo λ 10X/0.45 or 20X/0.75 objectives with a 0.7x demagnifier and Nikon Eclipse Ti microscope with a Flir, Chameleon3 CMOS camera. Longterm imaging was performed using a Carl Zeiss Plan-Apochromat 10x/0.45 objective with a 0.63x demagnifier and Carl Zeiss Axiovert 200m microscope also with a Flir, Chameleon3 camera housed inside an incubator for stable environmental control. All imaging was accomplished using custom automated software written using MATLAB and Micro-Manager. Image acquisition software is available on the GitHub repository: https://github. com/wollmanlab/Scope.
Automated image analysis was accomplished using custom-written software written in MATLAB. Image analysis software is available from the GitHub repository: https://github.com/wollmanlab/SingleCellVirusProcessing.
Automated image analysis was accomplished using custom-written software written in MATLAB. Image analysis software is available from the GitHub repository: https://github.com/wollmanlab/SingleCellVirusProcessing.
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