Ab180943

Manufactured by Abcam

Sourced in United States

Ab180943 is a primary antibody targeting the protein Rab9. It is suitable for use in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ab180943

Multiplex Protein Expression in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell lines used were as follows: HAE (CP-H209, Procell, Wuhan, China), A549 (CL-0016, Procell), HCC827 (CL-0094, Procell), NCL-H1299 (CL-0165, Procell), and NCL-H524 (CL-0403, Procell).
The following antibodies were used: anti-TMED2 (ab251705, Abcam, Cambridge, USA), anti-Ki-67 (ab15580, Abcam), anti-CEA (ab207718, Abcam), anti-NSE (ab180943, Abcam), anti-EGFR (ab200828, Abcam), anti-TLR4 (ab13556, Abcam), anti-NF-κB-p-p65 (BM3940, Boster, Wuhan, China), anti-IL-1β (ab2105, Abcam); anti-IL-18 (ab207323, Abcam); and anti-β-actin (M01263-2, Boster). The secondary antibodies used were anti-rabbit IgG (AS014, ABclonal, Wuhan, China) and anti-mouse IgG (H+L) (AS003, ABclonal).

Feng L., Cheng P., Feng Z, & Zhang X. (2022). Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinoma. World Journal of Surgical Oncology, 20, 32.

+ Open protocol

+ Expand

Protein Expression Analysis in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was lysed from cells using radioimmunoprecipitation assay buffer (RIPA buffer; Beyotime, Shanghai, China) supplemented with protease inhibitors. SDS/PAGE was used to separate 30 µg of total proteins, which were then transferred to a nitrocellulose membrane. The membranes were blocked with 5% non-fat milk and then incubated overnight at 4°C with specific primary antibodies. The membranes were washed and incubated with secondary antibodies at room temperature for 1 h. Electro chemiluminescent detection was used to visualize the protein bands. The expressions of GAPDH and β-actin were used as internal controls. The primary antibodies were used in this study: anti-SOX11 (Santa Cruz, CA, United States), anti-MYCN (#84406, Cell Signaling Technology, United States), anti-p21 (ab109199, Abcam, United States), anti-p27 (ab32034, Abcam, United States), anti-Cyclin D1 (ab134175, Abcam, United States), anti-NSE (ab180943, Abcam, United States), anti-CHGA (ab68271, Abcam, United States), and anti-SYP (ab32127, Abcam, United States). Quantification of the western blots was performed using ImageJ.

Ji S., Shi Y., Yang L., Zhang F., Li Y, & Xu F. (2022). miR-145-5p Inhibits Neuroendocrine Differentiation and Tumor Growth by Regulating the SOX11/MYCN Axis in Prostate cancer. Frontiers in Genetics, 13, 790621.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from cells in different groups after lysis with RIPA lysis buffer (Beyotime). Protein concentration was determined using a bicinchoninic acid assay (Thermo Fisher Scientific, USA). Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred onto PVDF membranes that were blocked with 5% milk powder containing 0.1% TBS-Tween-20, and incubated with specific primary antibodies against Bcl-2 (ab182858; Abcam, USA), cleaved caspase-3 (ab32042; Abcam), p53 (ab26; Abcam), LC3B (ab192890; Abcam), p62 (ab109012; Abcam), Beclin1 (ab210498; Abcam), TTF1 (#12373; CST, USA), TrkA (#2505; CST), TrkB (#4607; CST), NSE (ab180943; Abcam), MAP2 (ab32454; Abcam), TAU (ab76128; Abcam), and GAPDH (ab128915; Abcam) at 4°C overnight. After incubation with HRP-linked secondary antibodies (BA1051 and BA1054; BOSTER, China) for 2 h at room temperature, protein bands were visualized using chemiluminescence reagent (Millipore, USA) in a Bio-Rad Universal Hood II DOC Electrophoresis Imaging Cabinet.

Yang T., Li J., Zhuo Z., Zeng H., Tan T., Miao L., Zheng M., Yang J., Pan J., Hu C., Zou Y., He J, & Xia H. (2022). TTF1 suppresses neuroblastoma growth and induces neuroblastoma differentiation by targeting TrkA and the miR-204/TrkB axis. iScience, 25(7), 104655.

+ Open protocol

+ Expand

Quantification of BDEV Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from previously isolated BDEVs and the lungs, liver and kidney tissues of rats. The concentrations of BDEVs were determined by NTA and were adjusted to the same level in each group. The concentrations of protein samples obtained from the lung, liver and kidney were measured by using a BCA protein assay (Beyotime, Beijing, China). Proteins were loaded on SDS polyacrylamide gels(genscript, Nanjing, China), separated, and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). According to the reference interval of the antibody manual, the membranes were cut and respectively incubated with antibodies against GFAP (1:10000, ab7260, Abcam, Cambridge, UK), NSE (1:1000, ab180943, Abcam, Cambridge, UK), GAPDH (1:2500, ab9485, Abcam, Cambridge, UK), bax (1:1000, ab32503, Abcam, Cambridge, UK), bcl-2 (1:1000, ab32124, Abcam, Cambridge, UK), and cleaved caspase-3 (1:500, ab2302, Abcam, Cambridge, UK) overnight at 4 • C and then incubated with a HRP conjugated goat anti-rabbit IgG (1:1000; cat. no. 40295G; BIOSS). The membranes were exposed with the FlourChem FC3 Imaging System (proteinsimple, USA). Densitometric analysis was performed using ImageJ 7.0 software.

Lin H., Chen H., Qi B., Jiang Y., Lian N., Zhuang X, & Yu Y. (2021). Brain-derived extracellular vesicles mediated coagulopathy, inflammation and apoptosis after sepsis. Thrombosis research, 207.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!