Individual pools of 10x 3’ gene expression libraries were sequenced on Illumina’s Nextseq 2000 P3, Novaseq S2 and/or NovaSeq S4 flow cells with a targeted sequencing read depth of 20,000 reads per cell. Sequencing parameters were as follows: (1) for dual indexed libraries: Read 1=28 cycles, Index 1=10 cycles, Index 2=10 cycles, Read 2=90 cycles; (2) for single indexed libraries: Read 1=28 cycles, Index 1=8 cycles, Index 2=0 cycles, Read 2=91 cycles.
Nextseq 2000 p3
Manufactured by Illumina
The Nextseq 2000 P3 is a high-throughput sequencing system designed for a wide range of genomic applications. It utilizes Illumina's sequencing-by-synthesis technology to generate high-quality sequencing data. The system features a compact and efficient design, optimized for both research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nextseq 2000 p3
1
Single-cell RNA-seq Library Preparation
2
Single-cell RNA-seq Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Individual pools of 10x 3’ gene expression libraries were sequenced on Illumina’s Nextseq 2000 P3, Novaseq S2 and/or NovaSeq S4 flow cells with a targeted sequencing read depth of 20,000 reads per cell. Sequencing parameters were as follows: (1) for dual indexed libraries: Read 1=28 cycles, Index 1=10 cycles, Index 2=10 cycles, Read 2=90 cycles; (2) for single indexed libraries: Read 1=28 cycles, Index 1=8 cycles, Index 2=0 cycles, Read 2=91 cycles.
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