Jurkat (ATCC, Manassas, VA, USA), Jurkat NFAT (BPS Biosciences, San Diego, CA, USA), THP-1 NFKB (BPS Biosciences), and U937 (ATCC) cell lines were maintained in RPMI 1640 medium (Clontech, San Jose, CA, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, USA). RBL2H3 (ATCC) and HEK293 (ATCC) cell lines were maintained in DMEM medium (Clontech) supplemented with 10% fetal bovine serum. The Luva cell line (Kerafast, Boston, MA, USA) was maintained in StemPro-34 SFM medium supplemented with StemPro-34 Nutrient supplement and 2 m m L-glutamine (Gibco). All cells were cultured at 37°C, 5% CO2, and 95% humidity.
Dmem medium
Manufactured by Takara Bio
DMEM (Dulbecco's Modified Eagle's Medium) is a widely used cell culture medium that provides essential nutrients and growth factors for the in vitro cultivation of a variety of cell types. It is a basal medium that supports the growth and maintenance of cells in a controlled and standardized environment.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Takara Bio (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Jurkat, by American Type Culture Collection (1 mentions)
Rbl 2h3, by American Type Culture Collection (1 mentions)
Stempro 34 sfm medium, by Thermo Fisher Scientific (1 mentions)
2mml glutamine, by Thermo Fisher Scientific (1 mentions)
Stempro 34 nutrient supplement, by Thermo Fisher Scientific (1 mentions)
Mirna mimic, by Thermo Fisher Scientific (1 mentions)
Ambion mmessage mmachine, by Thermo Fisher Scientific (1 mentions)
3 protocols using dmem medium
1
Cell Culture Protocol for Cell Lines
2
Culturing Cell Lines and PBMCs
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Jurkat, U937, HL-60 cell lines and PBMCs were maintained in RPMI 1640 medium (Clontech) supplemented with 10% fetal bovine serum (FBS). RBL-2H3 cells (WT and M1) were cultured in DMEM medium (Clontech) with 10% fetal bovine serum (FBS). All cells were cultured at 37 °C, 5% CO2, and 95% humidity.
3
3T3 Fibroblast Transfection with Bcan 3'UTR and miR-9
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3T3 fibroblast were purchased from ATCC and grown in DMEM medium with tetracycline-free 10% FBS (Clontech). For transfection we used the Bcan 3′UTR plasmid or miR-9 GFP sensors (1 mg ml−1) along with miRNA mimics (Thermo Scientific; 500 nM each, Supplementary Table 4 ) and in combination with mCherry RNA. To generate mRNA for transfection, we used the pSLU plasmid64 (link) and the Ambion mMessage mMachine (AM1345; Ambion) according to manufacturer’s instructions.
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