Microtof control version 3

Manufactured by Bruker

Sourced in United States

The MicrOTOF control version 3.0 is a high-performance mass spectrometry control software from Bruker. It provides advanced data acquisition and analysis capabilities for Bruker's MicrOTOF series of mass spectrometers.

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Lab products found in correlation

Microtof 2, by Bruker (1 mentions) Dataanalysis 4, by Bruker (1 mentions) Maxis q tof, by Bruker (1 mentions) Proteinscape 3, by Bruker (1 mentions) Hystar 3, by Bruker (1 mentions)

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MicrOTOF Control 3.0 (2 citations)

2 protocols using microtof control version 3

Positive Mode Mass Spectrometry Protocol

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The mass spectrometer was a Bruker Daltonics micrOTOF II, equipped with electrospray (ESI) ion source. Compound spectra were obtained in the positive mode with direct infusion from an external syringe at a rate of 180 μL/hour. Nitrogen was used both as a nebulizer gas (pressure 0.4 bar) and a drying gas (gas flow 4.0 L/min). The drying temperature was 180 °C. The applied capillary voltage was 4500 V, the capillary exit was 150.0 V and skimmer 1 was set to 50.0 V. The transfer time of the ions from hexapole to orthogonal acceleration was 45 μs and hexapole radio frequency was 800.0 Vpp. Mass spectral data were collected from m/z 200 to 2000. TOF-MS was operated with micrOTOF control version 3.0 by Bruker Daltonics.

Benoit Y.D., Mitchell R.R., Wang W., Orlando L., Boyd A.L., Tanasijevic B., Aslostovar L., Shapovalova Z., Doyle M., Bergin C.J., Vojnits K., Casado F.L., Di Lu J., Porras D.P., García-Rodriguez J.L., Russell J., Zouggar A., Masibag A.N., Caba C., Koteva K., Kinthada L., Patel J.S., Andres S.N., Magolan J., Collins T.J., Wright G.D, & Bhatia M. (2021). Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics. Cell chemical biology, 28(10), 1394-1406.e10.

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Proteomic Analysis of Rat Proteins

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Protein spots were excised from the polyacrylamide gels and then analyzed as described in [17 (link),31 (link),32 (link)]. Briefly, after purification with STAGE-TIPs, peptide separation was achieved using a nano-LC device (Proxeon, Odense, Denmark) coupled to a maXis Q-TOF (quadrupole-time of flight) mass spectrometer with ultra-high resolution (Bruker Daltonics, Bremen, Germany). Appropriate software was used (HyStar 3.2 and MicroTOF control Version 3.0., ProteinScape 3.0 and DataAnalysis 4.0 (Bruker Daltonics, Billerica, MA, USA)) for data analysis. Only significant hits (MASCOT score ≥80 for proteins; ≥30 for peptides) were accepted. Proteins were identified by correlating tandem mass spectra with the UniProt/Swiss-Prot database (taxonomy = Rattus norvegicus). The MASCOT online search engine (http://www.matrixscience.com) was used. All nLC-MS/MS analyses were performed in duplicates (two samples per spot).

Ujcikova H., Eckhardt A., Hejnova L., Novotny J, & Svoboda P. (2021). Alterations in the Proteome and Phosphoproteome Profiles of Rat Hippocampus after Six Months of Morphine Withdrawal: Comparison with the Forebrain Cortex. Biomedicines, 10(1), 80.

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