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3 protocols using bindzil 40 130

1

Biomimetic Lipid Membrane Fabrication

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The lipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
(POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
(POPG), and N-(lissamine rhodamine B sulfonyl)-1,2
dioleoyl-sn-glycero-3-phosphoethanolamine (LRB-DOPE,
λexcem = 560/580 nm) were purchased
from Avanti Polar Lipids Inc. (Alabaster, AL). Sodium chloride (NaCl),
sodium dodecyl sulfate (SDS), dimethyl sulfoxide (DMSO), (3-glycidyloxypropyl)trimethoxysilane
(GOPS), tetraethyl orthosilicate (TEOS, 98%), poly(ethylene glycol)20–poly(propylene glycol)70–poly(ethylene
glycol)20 (P123), hydrochloric acid (HCl, 34%), acetone,
2-propanol (IPA), and chloroform (CHCl3) were purchased
from Sigma-Aldrich, Germany. Phosphate buffered saline (PBS) was prepared
from tablets (NaCl 137 mM, KCl 2.7 mM, phosphate buffer 10 mM, pH
7.4), also from Sigma-Aldrich. Poly(3,4-ethylenedioxythiophene)–poly(styrenesulfonate)
(PEDOT:PSS) aqueous solution (Clevios PH1000) was purchased from Heraeus
Precious Metals GmbH, Germany. Ethanol (99.7%) was purchased from
Solveco. Borosilcate coverslips (ø 25 mm, No. 2) were purchased
from VWR and QCM-D crystals from Q-Sense, Sweden. Bindzil 40/130 was
a gift from Akzo Nobel, Sweden.
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2

Silica Nanoparticle Hydrogel Formation

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An aggregated
silica particle hydrogel was formed from a filtered 9 wt % (4.1 vol
%) colloidal suspension of amorphous silica nanoparticles (Bindzil
40/130; AkzoNobel, PPC AB, Bohus, Sweden).39 (link),40 (link) In short, the gelation process was triggered by adjusting the pH
from 9.1 to 7.8 and by adding NaCl (s) to a final concentration of
0.5 M NaCl (aq). The gel was left to set for 2 weeks before electron
microscopy preparation.
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3

Silica-based Polymer Nanocomposite Synthesis

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Tetraethyl
orthosilicate (TEOS) ≥
99.0% and 2-propanol 99.5% were purchased from Aldrich (Milwaukee,
WI). (3-Aminopropyl)-triethoxysilane (APTES) 99%, hydrochloric acid
37%, and ammonium hydroxide solution 28–30% were purchased
from Sigma-Aldrich (St. Louis, MO). Monoglycidyl ether-terminated
poly(dimethylsiloxane) (PDMS-G) (Mw =
1000 and 5000 g mol–1) was purchased from Gelest
(Morrisville, PA). N-(2-aminoethyl-3-aminopropyl)methyldimethoxysilane
(Dynasylan 1411) ≥ 99.0% was obtained from Evonik (Marl, Germany).
PMMA granules were acquired from Arkema (VM100, i.e., a PMMA-co-EA polymer, ρ = 1.18 g cm–3)
(La Garenne-Colombes, France). Nanoparticles with diameters of 12
nm (Bindzil 40/220), 20 nm (Bindzil 40/130), and 60 nm (Levasil 50/50)
were a gift from AkzoNobel (Bohus, Sweden). These particles were dispersed
in aqueous solution and have surface-exposed silanol groups on the
surface as received. Absolute tetrahydrofuran (THF) was purchased
from Biosolve (Valkenswaard, The Netherlands). Ethanol absolute for
analysis was obtained from Merck (Darmstadt, Germany). Milli-Q water
was produced by a Millipore Synergy system (Billerica, MA). Unless
otherwise mentioned, all other chemicals were used as received.
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