Plan apochromat 63 na 1.46 oil immersion

Confocal images were acquired on a Zeiss LSM 780 microscope equipped with a Plan-Apochromat 63×/NA 1.46 oil immersion or a Plan-Apochromat 40×/NA 1.2 water objective (Zeiss AG, Oberkochen, Germany). The images were processed using the image-processing package Fiji (http://Fiji.sc/Fiji (accessed on 26 July 2021)) developed by Schindelin et al. [23 (link)] on the basis of ImageJ (http://imagej.nih.gov/ij (accessed on 26 July 2021)). In most figures, we show average projections of a series of confocal planes.

Confocal images were acquired on a Zeiss LSM 780 microscope equipped with a Plan-Apochromat 63×/NA 1.46 oil immersion or a Plan-Apochromat 40×/NA 1.2 water objective (Zeiss AG, Oberkochen, Germany). The images were processed using the image-processing package Fiji (http://Fiji.sc/Fiji) developed by Schindelin et al. (2012) (link) on the basis of ImageJ (http://imagej.nih.gov/ij). In the fluorescence images, we show average projections of series of confocal planes. The plane-to-plane distance was 0.1–0.2 µm. Since the RFP label bleaches at long periods of imaging, we corrected the signal in the red channel with the Bleach Correction plugin in Simple Ratio Mode. Bright fields show single-plane phase-contrast images. The contour lengths of the furrows in Fig. 2B and Fig. 6B were measured with the Segmented Line Tool. In Fig. 6C, the Polygon Selection Tool was used for the colour-coded contours of the whole cell at consecutive time points, and the substrate-attached areas were calculated using the Analyze and Measure Tool.