Penicillin rpmi media

Manufactured by Thermo Fisher Scientific

Sourced in United States

Penicillin RPMI media is a cell culture medium formulated for the growth of a variety of cell types. It contains the antibiotic penicillin to prevent bacterial contamination. The core function of this product is to provide a nutrient-rich environment to support the proliferation of cells in in vitro experiments.

Automatically generated - may contain errors

Lab products found in correlation

Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) X rad 320 biological irradiator, by Precision X-Ray (2 mentions) Foetal calf serum, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

RPMI media Penicillin

2 protocols using penicillin rpmi media

Bone Marrow Transplant and SRBC Immunization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recipient C57BL/6 Rag1^−/− mice 8–12 weeks old were irradiated with 425 cGy using an X-RAD 320 Biological Irradiator (Precision X-Ray, North Branford, CT, USA). Donor bone marrow was aspirated from femurs, humeri and tibia into B-cell medium comprising RPMI (Gibco, Carlsbad, CA, USA) with 10% heat-inactivated foetal calf serum (Gibco), 2 mMl-glutamine and 100 U/ml penicillin RPMI media (Gibco). At 15 h after irradiation, recipient mice were transplanted with an intravenous injection of 5–10 × 10⁶ bone marrow cells, comprising a mixture of 50% from CD45.1 congenic C57BL/6 mice and 50% from C57BL6 (CD45.2) mice that were either Lrba^−/− or Lrba^+/+.
Unmanipulated mice 8 weeks old, and bone marrow chimeras 8 weeks after marrow transplantation, were immunised with 2 × 10⁸ SRBCs given intravenously.

Burnett D.L., Parish I.A., Masle-Farquhar E., Brink R, & Goodnow C.C. (2017). Murine LRBA deficiency causes CTLA-4 deficiency in Tregs without progression to immune dysregulation. Immunology and Cell Biology, 95(9), 775-788.

+ Open protocol

+ Expand

Bone Marrow Transplantation in Transgenic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recipient mice of 8 to 12 wk of age were lethally irradiated (2 × 425 cGy) using an X-RAD 320 Biological Irradiator (Precision X-Ray). Femoral, humeral, and tibial bone marrow cells were aspirated into RPMI (Gibco) with 10% heat-inactivated fetal calf serum (FCS) (Gibco), 2 mM l-glutamine, and 100 U/mL penicillin RPMI media (Gibco). Fifteen hours after irradiation, recipient mice were transplanted with an i.v. injection of 5 to 10 × 10⁶ bone marrow cells. For mHEL^3X transgenic (CD45.2⁺) recipients, injected bone marrow cells were 80% of SW_HEL.Rag1^−/− (CD45.1⁺) origin and 20% of mHEL^3X transgenic (CD45.2⁺) origin. Control nontransgenic mice were reconstituted with less (45%) SW_HEL.Rag1^−/− (CD45.1⁺) and more (55%) wild-type (CD45.2⁺) bone marrow, so that comparable frequencies of anti-HEL B cells in the transitional subset of the spleen were obtained in chimeras irrespective of self-antigen (mHEL^3X) expression, as described previously (38 (link)). Chimeras were analyzed 8 to 14 wk after reconstitution.

Burnett D.L., Schofield P., Langley D.B., Jackson J., Bourne K., Wilson E., Porebski B.T., Buckle A.M., Brink R., Goodnow C.C, & Christ D. (2020). Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens. Proceedings of the National Academy of Sciences of the United States of America, 117(36), 22341-22350.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!