Reagents for buffer preparation were purchased from Fisher Scientific, unless otherwise specified. DNA primers were purchased from Eurofins MWG Operon. Chemically competent E.coli cells BL21(λDE3) were purchased from Lucigen or Novagen. QuikChange Lightning Site-Directed Mutagenesis kit was purchased from Agilent Technologies. Biotin-4-fluorescein was purchased from Biotium. Biotin-maleimide and streptavidin was purchased from Sigma Aldrich. Protease and phosphatase inhibitor mini tablets were purchased from Roche. For gene assembly, NEB builder kit was purchased from New England Biolabs. For Immunoblot, Opti-4CN detection kit was purchased from Biorad.
Biotin 4 fluorescein
Manufactured by Biotium
Biotin-4-fluorescein is a fluorescent dye conjugated to biotin. It is used as a probe for the detection and localization of biotinylated molecules in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (1 mentions)
Biotin maleimide, by Merck Group (1 mentions)
Protease and phosphatase inhibitor mini tablets, by Roche (1 mentions)
Neb builder kit, by New England Biolabs (1 mentions)
Opti 4cn detection kit, by Bio-Rad (1 mentions)
Dna primers, by Eurofins (1 mentions)
2 protocols using biotin 4 fluorescein
1
Recombinant Protein Expression Protocol
2
Biotinylated DNA Duplex Labeling Protocol
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Oregon green 488 Biocytin (lot 40300A, Fig 1 ) was purchased from Invitrogen (Eugene, OR). Avidin (CAS 1405-69-2, lot 608540) was purchased from Calbiochem (La Jolla, CA). HABA or 2-(4’-hydroxyazobenzene)-benzoic acid (CAS 1634-82-8, lot 52F-0073), streptavidin (CAS 9013-20-1), endoglycosidase H (CAS 37278-88-9) and d-biotin (CAS 58-85-5, lot 13F-3199) were all purchased from Sigma Aldrich (St. Louis, MO). Biotin-4-fluorescein (lot 31005, Fig 1 ) was purchased from Biotium, Inc. (Hayward, Ca). The 3’ end labeled fluorescein top strand with a modified biotinylated d-thymine at position 6 in the following sequence: 5’-GGGAA(biotin-dT)AACTTGGC*Fl-3’ (Fig 1 ) and the respective complement (5’-GCCAAGTTATTCCC-3’ ) were made by Tri-Link Biotechnologies, Inc. (San Diego, CA), and were both HPLC and PAGE purified. The sequences retain the G/C (base pairs) ends and fluorescein identical to those characterized extensively in our previous studies [42 (link), 44 (link), 46 (link)]. The biotinylated 14mer duplex (B7-DNAds*Fl) was formed with 5-10X excess complement and incubated for at least 20 min.
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